摘要
We tried to determine the optimum salinity for a cultured of olive flounder (Paralichthys olivaceus) by investigating after exposing the fish at different salinity (10, 15, 20 and 25 psu) for 24 and 48 hours compared with control group (fish before transfer to experimental tank). As a control groups, we compared an analyzed with other experimental groups using olive flounder in natural sea water. Hematological parameters including hematocrit (Ht) and hemoglobin (Hb), cortisol and glucose, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), $NH_3$, osmolality, total protein (TP), $Na^+$, $K^+$ and $Cl^-$ mostly exhibited significant changes at 10 and 15 psu groups compared with control groups for 24 and 48 hours exposed. Plasma SOD (superoxide dismutase) and CAT (catalase) activity also increased with experimental groups (10 and 15 psu) compared to the control groups. The expression of HSP70 mRNA was also higher at low-salinity (10 and 15 psu) than at control group. In particular, after 24 hours exposed, it expression to 15 psu groups showed a significant difference compared to the control group. However, after 48 hours exposed, it expression was higher in the 10 psu groups than the control. It is assumed that the changes in the hematological responses and hormone, homeostasis and metabolism were resulted in to protect fish body from stress. Based on these results, we are expected that it will be used as basic data for the culture of olive flounder prepared for low salinity.
摘要译文
我们尝试通过在不同盐度（10,15,20和25 psu）下暴露24小时和48小时后的鱼进行调查来确定橄榄比目鱼（Paralichthys olivaceus）的最佳盐度，与对照组（转移前的鱼）相比实验坦克）。作为对照组，我们将分析与使用天然海水中的橄榄牙鲆的其他实验组进行了比较。血液学参数包括血细胞比容（Ht）和血红蛋白（Hb），皮质醇和葡萄糖，天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT），$ NH_3 $，渗透压，总蛋白（TP），$ Na ^ + $，$ K ^ + $和$ Cl ^ - $在暴露24和48小时时，与对照组相比，在10和15 psu组中大多表现出显着变化。与对照组相比，实验组（10和15psu）的血浆SOD（超氧化物歧化酶）和CAT（过氧化氢酶）活性也增加。低盐度（10和15 psu）时HSP70 mRNA的表达也高于对照组。特别地，在暴露24小时后，与对照组相比，其表达至15psu组显示出显着差异。然而，暴露48小时后，10psu组的表达高于对照组。假设血液学反应和激素，体内平衡和代谢的变化导致保护鱼体免受压力。基于这些结果，我们预计它将被用作为低盐度制备的橄榄比目鱼养殖的基础数据。
Park, Hyung-Jun;Min, Byung-Hwa;. Stress Responses of Olive Flounder (Paralichthys olivaceus) to Salinity Changes[J]. Korean Journal of Ichthyology 한국어류학회지, 2018,30(1): 1-8