摘要
Two different extenders were compared for their effects on preservation of semen from Indigenous rams and on pregnancy rate (PR) in Indigenous ewes. Semen was collected from nine Indigenous rams (Ovis aries) once a week using an artificial vagina. Each ejaculate was divided into 2 aliquots, diluted with either commercial (Triladyl®) or locally manufactured (tris, fructose, citric acid, egg yolk: TFE, prepared in own laboratory) extenders and kept at 4°C for 48 h. Motility, viability, functional integrity and morphological changes were evaluated at 0, 24 and 48 h. Synchronized oestrus ewes inseminated transcervically with 24 and 48 h of preserved chilled semen diluted with Triladyl and TFE extenders separately. Semen preserved in Triladyl had better motility, viability, and functional integrity at 24 and 48 h (P < 0.001) than did in TFE. The morphologically normal spermatozoa up to 48 h of preservation did not differ between extenders. However, in abnormalities studied, Triladyl had detrimental effect on sperm acrosome and TFE on sperm tail (P < 0.001) at 24 and 48 h of preservation. But, midpiece was not affected by any extender (P > 0.05) over the entire period of preservation. The quality of semen decreased (P < 0.001) with increasing preservation time for both extenders. The extenders did not differ (P > 0.05) the overall PR after transcervical AI (TCAI) in ewes. Increased preservation time (48 h) negatively affected the PR in TFE extended semen compared with than that of Triladyl. The results suggest that the quality of chilled semen (motility, viability, and functional integrity) is more improved when preserved in Triladyl than if extended with a TFE. PR may higher when TCAI is performed with chilled semen preserved in Triladyl for a longer time than TFE. However, TFE extender may be used to dilute the semen for chilling and used in TCAI to get similar PR of Triladyl up to 24 h of preservation.
摘要译文
比较两种不同的扩增剂，以它们对来自土着rams和土着母羊的妊娠率（Pr）的精液保存的影响。使用人工阴道，从九个土着rams（ovis白羊座）收集精液。将每次射精分为2个等分试样，用商用（Triladyl®）或本地制造的（Tris，果糖，柠檬酸，蛋黄：TFE，在自己的实验室制备）中，并在4℃下保持48小时。在0,24和48小时评估运动，活力，功能完整性和形态学变化。同步的雌期性肌肌经，用24和48小时分别用三烷基和TFE扩展器稀释24和48小时。在三叶酰基中保存的精液具有比TFE中的24和48小时（P <0.001）的更好的运动，活力和功能完整性。延伸剂之间的形态学上正常的精子高达48小时没有差异。然而，在研究异常中，三烷基对精子毒物体和TFE在24和48小时的保存时对精子尾剂和TFE进行了不利影响。但是，在整个保存期间，中药不受任何增量剂（P> 0.05）的影响。液体的质量下降（P <0.001），增加了两个增量剂的保存时间。 eWES在母弓羊术AI（TCAI）之后的总PR没有差异（p> 0.05）。增加的保存时间（48小时）对TFE延长精液中的PR产生负面影响，而不是三烷基。结果表明，当在三丙基中保存时，更加改善的冷冻精液（运动，活力和功能完整性）的质量比如果用TFE延伸，则更加改善。当TCAI用在三丙基中保存的冷冻液比TFE保持较长的时间时，PR可能更高。然而，TFE扩充剂可用于稀释精液以进行冷却，并用于TCAI，以获得类似于24小时的三烷基的类似PR。
A. Rekha;B.F. Zohara;F.Y. Bari;M.G.S. Alam. Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep[J]. Animal Reproduction, 2016,13(4): 735-742