期刊文献

Field evaluation of a technique for transcervical intrauterine insemination of ewes 收藏

母羊经宫腔内人工授精技术的现场评估

原文求助发布源

作者

G.W.Halbert[1];H.Dobson[2];J.S.Walton[3];P.Sharpe[4];B.C.Buckrell[1];

作者单位

[1]Department of Population Medicine University of Guelph Guelph, Ontario Canada N1G 2W1[2]Department of Clinical Studies Ontario Veterinary College University of Guelph Guelph, Ontario Canada N1G 2W1[3]Department of Animal and Poultry Science University of Guelph Guelph, Ontario Canada N1G 2W1[d]New Liskeard College of Agricultural Technology New Liskeard, Ontario Canada

关键词

transcervical; intrauterine insemination; trial ewes;

关键词译文

宫颈;宫腔内人工授精;试验母羊;

发布日期

1 June 1990

页码

1231-1243

DOI

10.1016/0093-691X(90)90041-Q

来源信息

Theriogenology ISSN：0093-691X, 1990年, 33卷, 6期, 1231-1243页

摘要

In this study, three groups of ewes were inseminated with a transcervical intrauterine technique in order to evaluate the suitability of the technique for a commercial artificial insemination (AI) program using frozen semen. The ewes were synchronized into estrus with vaginal pessaries and pregnant mare serum gonadotrophin (PMSG) and were inseminated between 48 and 55 h following pessary removal. In Flock I, maiden and multiparous ewes (n = 12) received fresh semen containing 400 × 10⁶ spermatozoa. Flock II was divided accordingly: 40 multiparous ewes were inseminated with frozen semen using the transcervical technique and 40 ewes were inseminated laparoscopically with frozen semen. In Flock III, 38 multiparous ewes were inseminated transcervically with frozen semen containing 150 × 10⁶ spermatozoa. Pregnancy diagnosis was based on blood progesterone analysis 18 d following breeding, and real time ultrasound examination at 40 d. Pregnancy rates were 50, 50 and 68%, respectively, for the three groups inseminated transcervically and 70% for the ewes inseminated laparoscopically. The lambing rates were 50, 55 and 40%, respectively, for the ewes inseminated transcervically and 65% for the ewes inseminated laparoscopically. Lambing results were confirmed by matching the breeding dates to the lambing dates. These results demonstrate that the described technique is suitable for further evaluation using frozen semen in a commercial sheep AI program.

摘要译文

在这项研究中，为了评估该技术对使用冷冻精液的商业人工授精（AI）计划的适用性，对三组母羊进行子宫内宫腔技术的授精。母羊与阴道子宫托和妊娠母马血清促性腺激素（PMSG）同步进入发情期，并在子宫切除后48至55小时内进行授精。在Flock I中，处女和多胎母羊（n = 12）接受含有400×10 ^{6 精子的新鲜精液。因此将Flock II分开：使用经宫颈技术将40只母羊用冷冻精液授精，并用冷冻精液对40只母羊进行腹腔镜检查。在Flock III中，38只多胎母羊在子宫内用含有150×10 ^{6 精子的冷冻精液进行授精。妊娠诊断基于育种后18天的血液孕酮分析，以及40天的实时超声检查。对于经宫颈授精的三组和腹腔镜授精的母羊，妊娠率分别为50％，50％和68％。对于经宫颈授精的母羊，产羔率分别为50％，55％和40％，而腹腔镜检查的母羊产羔率为65％。通过将育种日期与产羔日期相匹配来确认产羔结果。这些结果表明，所描述的技术适合于在商业绵羊AI程序中使用冷冻精液进一步评估。}}

G.W.Halbert[1];H.Dobson[2];J.S.Walton[3];P.Sharpe[4];B.C.Buckrell[1];. Field evaluation of a technique for transcervical intrauterine insemination of ewes[J]. Theriogenology, 1990,33(6): 1231-1243