摘要
This study comprising of two trials was undertaken in ewes exhibiting natural oestrus to evaluate a simple transcervical artificial insemination technique suitably modified for use in tropical sheep. Twenty four adult multiparous Malpura ewes which had lambed 6 to 12 months prior to the experiment were utilized for two oestrous cycles during the autumn breeding season. The cervical penetration (n=47) was conducted in both first (n=24) and second (n=23) trial ≤6 to 18 h following onset of oestrus, while lambing rate was assessed in the second trial (n=23) following deposition of frozen-thawed semen having >70% mean post-thaw motility at os, mid cervix or uterus. The overall success achieved in cervical penetration was 44.7%. There was no difference in cervical penetration rate at ≤6 and 18 h after detection of oestrus (46.1% vs. 42.8%, respectively). A mean lambing rate of 22.7% was recorded in ewes inseminated using this technique. Mid cervical and transcervical insemination with frozen semen resulted in similar lambing rate (28.5 vs. 22.7%, respectively). However, no lambing was achieved on os-cervical insemination. Further efforts are needed to determine factors controlling the cervical penetration and conception rate following artificial insemination with frozen semen.
摘要译文
这项包含两项试验的研究是在表现出天然发情的母羊中进行的，以评估适用于热带绵羊的简单的经宫颈人工授精技术。在秋季繁殖季节期间，在实验前6至12个月产羔的24只成年多产马尔普拉母羊被用于两个发情周期。宫颈穿透（n = 47）在发情后≤6至18小时的第一次（n = 24）和第二次（n = 23）试验中进行，而在第二次试验中评估产羔率（n = 23）在口腔，子宫颈或子宫中具有> 70％平均解冻后运动的冻融精液沉积之后。宫颈穿透的总体成功率为44.7％。检测发情后≤6和18小时宫颈渗透率无差异（分别为46.1％和42.8％）。使用该技术授精的母羊的平均产羔率为22.7％。用冷冻精液进行中颈椎和经宫颈授精导致相似的产羔率（分别为28.5％和22.7％）。然而，在子宫颈人工授精中没有实现产羔。需要进一步努力以确定在用冷冻精液进行人工授精后控制宫颈穿透和受孕率的因素。
S.M.KNaqvi;AJoshi;SBag;S.RPareek;J.PMittal;. Cervical penetration and transcervical AI of tropical sheep (Malpura) at natural oestrus using frozen-thawed semen[J]. Small Ruminant Research, 1998,29(3): 329-333