摘要
Next generation sequencing tools have revolutionized analysis of the rumen microbiome, but it is impossible to make equivalent predictions about the impact of microbial popula-tions on host animal energy status solely based on their qualitative presence in the rumen, or their quantitative population numbers alone. To evaluate the rumen microbial popula-tion’s effect on cattle production there must be a holistic understanding of the microbial ecosystem trophic niches, degradative activities, biosynthetic pathways, populations, and interactions between feedstuffs, ruminal microbes, and the ruminant animal. An evaluation of the association between the ruminal microbiota fermentation and cattle feed efficiency was conducted utilizing DNA sequencing. Although the sample size was small, findings suggested more efficient steers in the feedlot stage had ruminal microbial populations that were more involved in fiber degradation. A second study was conducted to assess the role of Non-Protein Nitrogen (NPN) on the in vitro mixed ruminal microorganism fermentation.
摘要译文
下一代测序工具已经彻底改变了瘤胃微生物组的分析方法，但是不可能仅基于瘤胃中的定性存在或仅根据其定量种群数量就微生物种群对宿主动物能量状态的影响做出同等的预测。要评估瘤胃微生物种群对牛生产的影响，必须全面了解微生物生态系统的营养位，降解活性，生物合成途径，种群以及饲料，瘤胃微生物和反刍动物之间的相互作用。利用DNA测序对瘤胃微生物群发酵与牛饲料效率之间的关联进行评估。尽管样本量很小，但研究结果表明，在育肥阶段，更有效的ste牛皮瘤胃微生物种群更多地参与了纤维降解。进行了第二项研究，以评估非蛋白质氮（NPN）在体外混合瘤胃微生物发酵中的作用。
Carmichael, Mia Nicole. The Application of DNA Sequencing Technologies as an Aid in the Metaanalysis of the Ruminal Microbiome and Beef Steers from a Commercial Feedlot[D]. US: University of Georgia, 2020