摘要
The aim of this study was to investigate the post-thaw motility and morphological defect rates of cat semen frozen with various extenders, and pregnancy rates after intrauterine insemination (IUI). Cats, 2 males and 14 queens, 2 to 3 years of age, and under the same managemental conditions were used. Semen was collected by means of an electro-ejaculator under general anesthesia. Based on spermatological traits, semen samples presumed suitable for freezing were extended with either tris-fructose-citric acid (EY-TFC) containing 20% egg yolk, or skimmed milk-glucose-taurine (EY-SMGT) containing 10% egg yolk extender, and frozen in 0.25 mL straws. For the extenders, post-thaw motility and morphological abnormality values were found to be efficient for IUI and no statistical significance was observed between the values of the extender groups. To achieve ovulation, queens showing behavioral estrus received 250 IU of hCG via IM route, 30 h prior to IUI. Under general anesthesia, queens were laparatomised on the medial line and the left and right uterine horn were taken out. Into both uterine horns, 2 straws containing 25 × 106 sperm each were thawed at 37 °C for 30 s and equally injected. Pregnancy was diagnosed using an ultrasonography device on the 19th day after IUI by detecting the embryonic vesicles. Of the 3 EY-TFC group queens found to be pregnant, 1 aborted and other 2 had early embryonic death (EED); the pregnancy rate was 42.90%. In a queen in the EY-SMGT group, no ovulation took place after the hCG injection. In 2 of the queens found to be pregnant in this group, EED occurred and the pregnancy rate was 33.30%. In conclusion, cat semen extended and frozen with EY-TFC and EY-SMGT extenders was found to be successful in post-thaw motility and morphological defect rates. Therefore, surgical IUI may be more effective for feline AI using frozen semen rather than intravaginal insemination.
摘要译文
这项研究的目的是探讨宫腔内人工授精（IUI）后解冻后活力和精液猫形态缺陷率冻结与各种扩展和怀孕率。猫，2名男性和14王后，2至3岁，并且在同一managemental的条件下被使用。精液收集在全身麻醉下的电 - ejaculator的手段。基于spermatological性状，推定适合冷冻精液样本进行了扩展与含有任一三 - 果糖 - 柠檬酸（EY-TFC）的20％的蛋黄，或脱脂牛奶，糖，牛磺酸（EY-SMGT）含10％蛋黄扩展，并冻结在0.25毫升吸管。对于扩展，解冻后的活力和形态学异常值被认为是有效的IUI和增量剂组的值之间没有观察到统计学显着性。为了达到排卵，皇后呈现发情行为收到250 IU的hCG通过IM途径，之前IUI 30小时。在全身麻醉下，皇后被laparatomised上内侧线和左，右子宫角取出。到两个子宫角，2吸管含有25×106精子各解冻，在37℃下30秒和同样喷射。使用上IUI后19天的超声装置通过检测胚胎囊怀孕被诊断。发现怀孕3 EY-TFC组王后，1中止和其他2例胚胎早期死亡（EED）;怀孕率为42.90％。在EY-SMGT组中的女王，无排卵的绒毛膜促性腺激素注射后发生。在2发现怀孕了本组的皇后，EED发生的妊娠率为33.30％。结论，延伸并与EY-TFC和EY-SMGT增量冷冻精液猫被发现是成功的在解冻后活力和形态学缺陷率。因此，外科IUI可能更有效地使用猫人工智能冷冻精液而非阴道内授精。
Alper BARAN[1]; Çağatay TEK[2]; Kamber DEMİR[1]; Ahmet SABUNCU[2]; Özen Banu ÖZDAŞ[1]. Intrauterine insemination with cat semen frozen with various extenders[J]. Turkish Journal of Veterinary & Animal Sciences, 2011,35(5 October p. 281-373): 311-318