期刊文献

Specific-detection of alkaline phosphatase activity in individual species of marine phytoplankton 收藏

在海洋浮游植物的个别物种特异性检测碱性磷酸酶活性
原文求助 发布源
作者
Haruo Yamaguchi[1]; Mineo Yamaguchi[1];Masao Adachi[2]
作者单位
[1]Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency;[2]Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University
关键词
alkaline phosphatase; ELF; marine phytoplankton; Karenia mikimotoi; Skeletonema costatum
关键词译文
碱性磷酸酶; ELF;海洋浮游植物;米氏凯伦藻;中肋骨条藻
页码
214-217
DOI
10.3800/pbr.1.214
来源信息
Plankton and Benthos Research ISSN：1880-8247, 2006年, 1卷, 4期, 214-217页
摘要
We evaluated the suitability of ELF-based assay (ELF: Enzyme-Labeled Fluorescence, Molecular Probes) as a tool for specific-detection of alkaline phosphatase (AP) activity in individual species of marine phytoplankton. Among 4 species of marine phytoplankton tested in the present culture experiments, the ELF-labeled cells of Karenia mikimotoi (Dinophyceae) and Skeletonema costatum (Bacillariophyceae) were simultaneously found with AP activity that was determined by using 4-methylumbelliferyl phosphate under the phosphorus-starved state but not found under the phosphorus-replete state. There were significant positive correlations between the percentage of ELF-labeled cells and AP activity of K. mikimotoi and of S. costatum. In contrast for Chattonella antiqua and Heterosigma akashiwo (Raphidophyceae) which do not produce AP, no ELF-labeled cells were found even under the phosphorus-starved state. Thus, ELF-based assay is considered to be useful for specific-detection of AP activity.
摘要译文
我们评估了基于ELF的测定法的适用性（ELF：酶标记的荧光，分子探针）作为用于特定检测海洋浮游植物的个别物种碱性磷酸酶（AP）活性的工具。其中4种海洋浮游植物在目前的培养实验测试了，米氏凯伦藻（甲藻纲）和中肋骨条藻（硅藻）的ELF标记的细胞同时被使用在磷饥饿4-甲基磷酸酯确定AP活动中状态，但磷充满的状态下，没有找到。有ELF标记细胞的米氏凯伦藻的比例和AP活性之间的中肋骨条藻和显著的正相关关系。与此相反的卡盾藻蝇和赤潮异弯藻（Raphidophyceae）不产生AP，没有发现ELF标记的细胞甚至是磷饥饿状态下。因此，ELF为基础的检测被认为是对于特定检测AP活性是有用的。
Haruo Yamaguchi[1]; Mineo Yamaguchi[1];Masao Adachi[2]. Specific-detection of alkaline phosphatase activity in individual species of marine phytoplankton[J]. Plankton and Benthos Research, 2006,1(4): 214-217