摘要
Bone necrosis secondary to radiation was previously attributed to trauma of devitalized bone and microbiological sepsis. However, conventional microbiological technique has failed to demonstrate microorganisms throughout osteoradionecrotic bone, claimed to be hypoxic, hypovascular and hypocellular. The aim of the present study was to examine such bone for bacteria using DNA– DNA hybridization. Compared to standard culture methods this technique enables the investigation of a vast number of bacteria in a fairly short time.
Twelve deep medullary specimens from resected radionecrotic mandibles were studied. A multitude of bacterial species were detected, most of them anaerobic. Porphyromonas gingivalis was the most predominant organism, followed by Fusobacterium nucleatum subspecies polymorphum. All samples contained Actinomyces, Prevotella and F. nucleatum. The results of this study indicate that bacteria, particularly anaerobes, may play a more fundamental role in the pathophysiology of osteoradionecrosis than being merely surface contaminants.
摘要译文
继发于放射线的骨坏死先前归因于失活骨的创伤和微生物败血症。然而，传统的微生物技术未能证明整个骨质疏松症骨质中的微生物，声称是低氧，低血管和低细胞。本研究的目的是使用DNA-DNA杂交检查这种细菌骨。与标准培养方法相比，该技术能够在相当短的时间内研究大量细菌。研究了来自切除的放射性坏死下颌骨的12个深髓质标本。检测到大量细菌物种，其中大多数是厌氧的。牙龈卟啉单胞菌是最主要的生物体，其次是Fusobacterium nucleatum亚种多形体。所有样品均含有放线菌，普氏菌和F. nucleatum。这项研究的结果表明，细菌，特别是厌氧菌，可能在骨质疏松症的病理生理学中发挥比仅仅是表面污染物更重要的作用。
G.Støre[1];E.R.K.Eribe[2];I.Olsen[2];. DNA–DNA hybridization demonstrates multiple bacteria in osteoradionecrosis[J]. International Journal of Oral and Maxillofacial Surgery, 2005,34(2): 193-196