摘要
The conventional method of human oocyte maturation in vitro in the presence of gonadotrophins continues to be a relatively low-success procedure in the assisted conception programme owing to suboptimal maturation conditions in the absence of an ovarian ‘niche’ and poor understanding of this procedure at the molecular level in oocytes. In this study, the gene expression profiles of human oocytes were analysed according to their manner of maturation: in vivo (in the ovaries) or in vitro (matured either by the conventional method or by a new approach – co-cultured with cumulus cells of mature oocytes from the same patient). Our results show that the in-vitro maturation procedure strongly affects the gene expression profile of human oocytes, including several genes involved in transcriptional regulation, embryogenesis, epigenetics, development, and the cell cycle. The in-vitro maturation of oocytes co-cultured with cumulus cells from mature oocytes provides an ovarian ‘niche’ to some degree, which improves oocyte maturation rates and their gene expression profile to the extent that they are more comparable to oocytes that naturally mature in the ovarian follicle.
摘要译文
由于在缺乏卵巢“生态位”的情况下成熟条件不理想，并且在卵母细胞的分子水平上对这一程序的理解不够，所以辅助受孕程序中的程序。在这个研究中，eir成熟方式：体内（在卵巢中）或体外（通过常规方法或通过新方法成熟 - 与来自同一患者的成熟卵母细胞的卵丘细胞共培养）。我们的研究结果表明，体外成熟过程强烈影响人类卵母细胞的基因表达谱，包括涉及转录调控，胚胎发生，表观遗传学，发育和细胞周期。与来自成熟卵母细胞的卵丘细胞共培养的卵母细胞的体外成熟在一定程度上提供卵巢“生态位”这提高了卵母细胞成熟率及其基因表达谱，使其与卵巢卵泡中天然成熟的卵母细胞更相似。
IrmaVirant-Klun[a];ChrisBauer[b];AndersStåhlberg[c];MikaelKubista[d];ThomasSkutella[e];. Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes[J]. Reproductive BioMedicine Online, 2018,36(5): 508-523