摘要
A regression approach was applied to determine the influence of feed provisioning prior to digesta sampling on precaecal (pc) amino acid (AA) digestibility in broiler chickens. Soybean meal was used as an example test ingredient. Five feed-provisioning protocols were investigated, four with restricted provision and one with ad libitum provision. When provision was restricted, feed was provided for 30 min after a withdrawal period of 12 h. Digesta were sampled 1, 2, 4 and 6 h after feeding commenced. A diet containing 300 g maize starch/kg was prepared. Half or all the maize starch was replaced with soybean meal in two other diets. Average pc digestibility of all determined AA in the soybean meal was 86% for the 4 and 6-h protocols and 66% and 60% for the 2 and 1-h protocols, respectively. Average pc AA digestibility of soybean meal was 76% for ad libitum feed provision. Feed provisioning also influenced the determined variance. Variance in digestibility ranked in magnitude 1 h > ad libitum > 2 h > 6 h > 4 h for all AA. Owing to the considerable influence of feed-provisioning protocols found in this study, comparisons of pc AA digestibility between studies applying different protocols prior to digesta sampling must be treated with caution. Digestibility experiments aimed at providing estimates for practical feed formulation should use feed-provisioning procedures similar to those used in practice.
摘要译文
采用回归方法确定消化物取样前饲料供应对肉鸡预饲料(pc)氨基酸(AA)消化率的影响。大豆粉被用作实例测试成分。调查了五个饲料供应协议,其中四个供应有限,另一个供应随意。当提供限制时,在12小时的停药期后提供饲料30分钟。饲养开始后1,2,4和6小时采集消化液。制备含有300g玉米淀粉/ kg的饮食。在其他两种饮食中,一半或全部玉米淀粉被豆粕代替。对于4和6小时方案,大豆粉中所有测定的AA的平均pc消化率分别为86%和2小时和1小时方案的66%和60%。自由采食饲料中豆粕的平均pc AA消化率为76%。 Feed配置也会影响确定的方差。所有AA的消化率差异均为1小时随意 2小时 6小时 4小时。由于本研究中发现的饲料供应协议的影响很大,在消化物取样之前应用不同方案的研究之间的PC AA消化率的比较必须谨慎对待。旨在为实际饲料配方提供估计值的消化率实验应使用类似于实际使用的饲料供应程序。
Wolfgang Siegert[1];Christian Ganzer[2];Holger Kluth[2];Markus Rodehutscord[3]. Influence of feed provisioning prior to digesta sampling on precaecal amino acid digestibility in broiler chickens[J]. Archives of Animal Nutrition, 2018,72(3): 190-204