摘要
Cell-free extracts from M-l3 am5 infected Escherichia coli cells which are highly concentrated on cellophane membrane disks replicate efficiently endogenous M-13 duplex DNA. If the reaction is carried out in the presence of bromodeoxyuridine triphosphate, the majority of the label is found in two classes of hybrid DNA molecules in which either the viral or the complementary strand is newly synthesized. A minor portion of the label is incorporated into fully synthetic duplex DNA. DNA synthesis requires ATP and is inhibited by nalidixic acid, novobiocin, and arabinosylnucleoside triphosphates. Rifampicin blocks preferentially the synthesis of molecules with labeled complementary strands. A similar effect is observed upon addition of the helix-destabilising M-13 gene V protein. In contrast, addition of E. coli helix-destabilising protein (Eco HD-protein) stimulates the synthesis of both types of hybrid DNA molecules as well as the formation of fully synthetic duplex DNA.
摘要译文
来自M-13 am5感染的大肠杆菌细胞的无细胞提取物高度浓缩在玻璃纸膜盘上，有效地复制内源性M-13双链体DNA。如果反应在溴脱氧尿苷三磷酸存在下进行，则大部分标记存在于两类杂合DNA分子中，其中新合成了病毒或互补链。将标记的一小部分掺入全合成双链体DNA中。 DNA合成需要ATP并被萘啶酸，新生霉素和阿拉伯糖核苷三磷酸抑制。利福平优先阻断具有标记的互补链的分子的合成。加入螺旋去稳定化M-13基因V蛋白后观察到类似的效果。相反，添加大肠杆菌螺旋去稳定蛋白（Eco HD-蛋白）刺激两种类型的杂合DNA分子的合成以及全合成双链DNA的形成。
Peter K. Schneck; Bob van Drop; Walter L. Staudenbauer; Peter Hans Hofschneider. A cell-free system for the replication of bacteriophage M-13 duplex DNA[J]. Nucleic Acids Research, 1978,5(5): 1689–1700