期刊文献

Molecular probe for identification of cysts of resting cyst of PSP-producer Alexandrium tamarense (Dinophyceae) 收藏

用于鉴定PSP-生产者塔玛亚历山大藻（Dinophyceae）静息囊肿囊肿的分子探针
原文求助 发布源
作者
Cho, Eun-Seob;
关键词
Alexandrium tamarense;Alexandrium catenella;biotoxin monitoring system;Chinhae Bay;DNA probe;red tide;resting cysts;
关键词译文
塔玛亚历山大藻（Alexandrium tamarense）;亚历山大藻（Alexandrium catenella）;生物毒素监测系统; Chinhae Bay; DNA探针;赤潮;静息囊肿;
页码
163-167
DOI
10.5352/JLS.2003.13.2.163
来源信息
Korean journal of life science ISSN：1225-9918, 2003年, 13卷, 2期, 163-167页
摘要
Identification of species within the toxin-producing genus Alexandrium is vital for biotoxin monitoring and mitigation decisions regarding shellfish industry. In particular, the discrimination of resting cysts of only A. tamarense from that of Alexandrium spp. is considerable important to fundamentally monitor and predict this species before vegetative cells occur in the nature. Fluorescent cTAM-F1 DNA probe was responsible to not only binding the activity of the vegetative cells in A. tamarense, but also to the resting cysts, which was treated with methanol after fixation and stained by primuline on the surface The location of fluorescence in cultured vegetative cells and resting cysts was almost at tile bottom of the nucleus. The optimal incubation temperature and time using in situ hybridization were 50- and 40-60 min, respectively, to penetrate the DNA probe into cell.
摘要译文
鉴定产生毒素的亚种的物种Alexandrium对于贝类工业的生物毒素监测和缓解决策至关重要。特别是，只有塔玛亚历山大藻的休眠孢囊与亚历山大藻的区别。在营养细胞出现在自然界之前，从根本上监测和预测这个物种是非常重要的。荧光cTAM-F1 DNA探针不仅与塔玛亚历山大藻营养细胞的结合有关，而且与固定的囊肿有关，固定后用甲醇处理，表面有普鲁卡因染色。培养荧光的位置营养细胞和静息囊肿几乎位于细胞核的底部。使用原位杂交的最佳温育温度和时间分别为50和40-60分钟，以穿透DNA探针进入细胞。
Cho, Eun-Seob;. Molecular probe for identification of cysts of resting cyst of PSP-producer Alexandrium tamarense (Dinophyceae)[J]. Korean journal of life science, 2003,13(2): 163-167