摘要
Three studies were conducted to determine the potential of experimental feed additives (EFAs), clays or non-digestible yeast oligosaccharides, to reduce milk aflatoxin (AFM1) concentrations in lactating Holstein cows consuming aflatoxin-contaminated diets. All studies included a pre-treatment period and a 2-week experimental period in a randomized block design. During the pre-treatment period, cows received a total mixed ration (TMR) with no aflatoxin contamination. During both experimental weeks, all cows were fed a TMR containing aflatoxin-contaminated corn. During experimental week 1, cows received no EFA’s in the TMR, but EFA’s were included in the TMR for the second experimental week. In studies 1 and 2, the experimental period consisted of 2 weeks each lasting 7 days with 12 cows per treatment. Aflatoxin M1 concentrations were analysed by HPLC for milk samples collected on days 5–7 and days 11–14. In various experiments, treatments included control (no EFA), 100 g/cow daily of experimental Lallemand® product, 10 g/cow daily of MTB-100®-2004, (Alltech, Inc.), 10 g/cow daily of MTB-100®-2006, (Alltech, Inc.), 10 g/cow daily of experimental Alltech® product (Alltech, Inc.) and 227 g/cow daily of Astra-Ben 20® (AB-20®; Prince Agri Products, Inc.). In study 3, the experimental period of 2 weeks each lasting 8 days and milk samples were collected from day 4 to 8 and day 11 to 16. Milk samples from study 3 were analysed for AFM1 concentrations by ELISA. For all experiments, changes in AFM1 concentrations because of the addition of EFA’s were calculated. Four of the five EFAs tested in this study had no significant effect on AFM1 concentrations. However, the addition of AB-20® resulted in a significant decrease in AFM1 concentrations (60.4%). In summary, the addition of AB-20® to the diet of cattle appears to be effective for significantly reducing AFM1 concentrations in the milk of cows fed an aflatoxin-contaminated diet.
摘要译文
三个研究被进行，以确定实验饲料添加剂（EFA可以），粘土或不可消化的酵母寡糖的潜力，降低牛奶中黄曲霉毒素（AFM 1）的浓度在哺乳期荷斯坦奶牛食用黄曲霉毒素污染的饲料。所有研究包括一个预处理周期和2周的实验期间在随机区组设计。在预治疗期间，母牛接受全混合日粮（TMR），无黄曲霉毒素污染。在这两个实验周，所有的奶牛饲喂含黄曲霉毒素污染的玉米一个TMR。在实验第1周，母牛接受无EFA的在TMR，但EFA的被列入在TMR为第二试验星期。在研究1和2，实验期间包括2周每期7天12头奶牛每待遇。黄曲霉毒素M 1的浓度用HPLC进行收集天5-7和11-14天的牛奶样品进行分析。在各种实验中，每天的治疗包括控制（无EFA），100克/牛实验拉勒曼德的SUPMTB-100为10克/牛每天SUP/燮（AB-20 SUP从研究3牛奶样品进行AFM 1浓度通过ELISA进行分析。对于所有的实验中，变化的AFM 1的浓度，因为除了EFA的的进行了计算。四五个必需脂肪酸在这项研究中测试了对AFM 1浓度没有显著的效果。然而，加入AB-20的SUP/ SUP导致了AFM 1浓度（60.4％）一显著下降。总之，除了AB-20的SUP/ SUP牛的饮食似乎是有效的显著减少的AFM 1浓度在奶牛的奶喂养的黄曲霉毒素污染的饮食。
L. Kissell[1,2]; S. Davidson[1]; B. A. Hopkins[1]; G. W. Smith[2]; L. W. Whitlow[1];. Effect of experimental feed additives on aflatoxin in milk of dairy cows fed aflatoxin-contaminated diets[J]. Journal of Animal Physiology and Animal Nutrition, 2013,97(4): 694-700