摘要
This study was conducted at the Nimbkar Agricultural Research Institute in Maharashtra, India to evaluate the feasibility of using the technique of laparoscopic intrauterine insemination in ewes under field conditions for the first time in India. Two groups of multiparous Patanwadi ewes were synchronized into oestrus, one group with vaginal sponges and the other with Controlled Internal Drug Release dispensers (CIDRs). Both groups were injected with 500 IU pregnant mare serum gonadotrophin (PMSG) and were inseminated with the laparoscopic intrauterine technique at 56–58 h after sponge/CIDR removal. The first group of 30 ewes was inseminated in January 1992 with fresh, diluted Awassi ram semen which was not pooled. The second group of 25 ewes was inseminated in June 1992 with fresh, undiluted Awassi ram semen which was not pooled. The conception rates were 77% and 72%, respectively and the lambing rates (percentage of ewes lambing) were 77% and 60% for the two groups. In addition, 35 Patanwadi ewes, some of which were the same as those in the first two groups, were cervically inseminated on natural oestrus with fresh, undiluted Awassi ram semen (not pooled) between October 1992 and October 1993. Similarly, 18 Patanwadi ewes on natural oestrus were naturally mated with Awassi rams during the same period. The conception rates were 69% and 83%, respectively and the lambing rates were 57% and 78% for the cervically inseminated and naturally mated ewes. The technique of laparoscopic intrauterine insemination in ewes was thus used successfully under primitive conditions.
摘要译文
这项研究是在马哈拉施特拉邦的Nimbkar农业研究所进行的，印度首次在印度评估在野外条件下使用腹腔镜宫腔内人工授精技术的可行性。两组多产的帕坦瓦迪母羊同步进入发情，一组带有阴道海绵，另一组带有受控内部药物释放分配器（CIDR）。两组均注射500 IU妊娠母马血清促性腺激素（PMSG），并在海绵/ CIDR去除后56-58小时用腹腔镜宫内技术进行授精。第一组30只母羊于1992年1月用新鲜稀释的Awassi ram精液进行了授精，这些精液未被合并。第二组25只母羊于1992年6月以新鲜的方式受精，没有稀释的Awassi ram精液没有汇集。受孕率分别为77％和72％，两组的产羔率（母羊产羔率）分别为77％和60％。此外，35只Patanwadi母羊，其中一些与前两组中的母羊一样，用新鲜的天然发情进行了子宫颈受精。1992年10月至1993年10月之间未稀释的Awassi ram精液（未汇集）。同样，在同一时期，18只Patanwadi母羊在自然发情时与Awassi公羊自然交配。受孕率分别为69％和83％，母系受精和自然交配的母羊产羔率分别为57％和78％。因此，在原始条件下成功地使用了母羊腹腔镜子宫内人工授精技术。
P.M.Ghalsasi[a];C.Nimbkar[b][∗];. Evaluation of laparoscopic intrauterine insemination in ewes[J]. Small Ruminant Research, 1996,23(1): 69-73