摘要
One hundred fifty-two bitches of seven breeds were vaginally inseminated with fresh or frozen-thawed semen of 10 stud dogs of respective breeds. The semen was supplemented with prostatic fluid before insemination. In experiment 1 bitches of each breed were randomly assigned to three treatment groups, consisting of 29 females (group 1), 33 females (group 2) and 32 females (group 3). In group 1 bitches were inseminated into vagina with fresh semen using a bovine infusion pipette. In group 2 bitches were inseminated into vagina with fresh semen using the Osiris catheter. In group 3 bitches were inseminated with frozen-thawed semen with the Osiris catheter. The number of sperms in each insemination dose was adjusted to 300 × 106. In experiment two bitches were randomly assigned to two treatment groups, consisting of 30 females (group A) and 28 females (group B). In group A bitches were inseminated with fresh semen, whereas in group B with frozen-thawed semen. Osiris catheter was used in both groups. The total number of sperms was adjusted to provide 250 × 106 of progressively motile spermatozoa in each insemination dose. In experiment 1 the pregnancy rates/whelping rates were 86.2/82.8%, 81.8/81.8% and 59.4/59.4% for groups 1, 2 and 3, respectively. The differences between group 1 and 3 were statistically significant (p < 0.05). The litter sizes at birth/litter sizes at weaning were 5.8 ± 2.3/5.4 ± 2.0, 6.3 ± 1.4/5.7 ± 1.0 and 3.9 ± 1.2/3.5 ± 1.5 in groups 1, 2 and 3, respectively. The litter size at birth and at weaning was reduced (p < 0.05) when frozen-thawed semen was used for insemination (group 3). There were not significant (p > 0.05) differences in the litter size between groups 1 and 2. In experiment 2 pregnancy rates/whelping rates and litter sizes at birth/litter sizes at weaning were 86.7/86.7%, 60.7/57.1% (p < 0.05) and 6.1 ± 1.6/5.7 ± 1.7, 4.0 ± 1.4/3.8 ± 1.4 (p < 0.05) in groups A and B, respectively. This study shows that results of AI with a fresh semen using a bovine infusion pipette and the Osiris catheter are equivalent. The results of the use of the Osiris catheter for vaginal insemination of frozen-thawed dog semen extended with prostatic fluid after thawing are not encouraging. The pregnancy rate, whelping rate and litter size are reduced when frozen-thawed, prostatic fluid-supplemented semen is vaginally deposited using the Osiris catheter.
摘要译文
一百五十二个七个品种的母犬用新鲜或冷冻解冻的各10个品种的种公狗的精液进行阴道授精。受精前精液补充前列腺液。在实验中，将每个品种的1只母犬随机分配到三个处理组，其中包括29只雌性（组1），33名女性（组2）和32名女性（组3）。在第1组中，母犬使用牛注入移液管以新鲜精液授精入阴道。第2组母犬使用Osiris导管以新鲜精液授精入阴道。在第3组中，母狗用Osiris导管用冷冻解冻精液授精。每次受精剂量的精子数量调整为300×106。在实验中，两只母犬被随机分配到两个处理组，由30名女性（A组）和28名女性（B组）组成。 A组母犬用新鲜精液进行授精，而B组用冷冻精液。两组均使用Osiris导管。调整精子总数以在每次授精剂量中提供250×106个逐渐运动的精子。在实验1中，怀孕率/怀孕率为86.2 / 82.8％第1组，第2组和第3组分别为81.8 / 81.8％和59.4 / 59.4％。组1和组3之间的差异具有统计学显着性（p 0.05）。在1,2和3组，分娩时出生仔数/窝产仔数分别为5.8±2.3 / 5.4±2.0,6.3±1.4 / 5.7±1.0和3.9±1.2 / 3.5±1.5。当冷冻精液用于授精时，出生和断奶窝产仔数减少（p 0.05）（第3组）。没有显着（p 0）。05）在第1组和第2组之间产仔数差异。在实验2中，怀孕率/产仔率和出生时产仔数/产仔数分别为86.7 / 86.7％，60.7 / 57.1％（p 0.05）和6。A组和B组分别为1±1.6 / 5.7±1.7,4.0±1.4 / 3.8±1.4（p 0.05）。这项研究表明，使用牛注入移液管和Osiris导管的新鲜精液AI结果是相同的。使用Osiris导管进行阴道人工授精的结果并不令人鼓舞，后者在解冻后用前列腺液延长。怀孕率，当冰冻融化时，排卵率和产仔数减少，使用Osiris导管辅助前列腺液补充精液阴道沉积。
WojciechNiżański;. Intravaginal insemination of bitches with fresh and frozen-thawed semen with addition of prostatic fluid: Use of an infusion pipette and the Osiris catheter[J]. Theriogenology, 2006,66(2): 470-483