摘要
Marbling from intramuscular fat is an important trait of meat quality and has an economic benefit for the beef industry. Quantitative trait loci (QTL) fine mapping was performed to identify the marbling trait in 266 Hanwoo steers using a 10K single nucleotide polymorphism panel with the combined linkage and linkage disequilibrium method. As a result, we found nine putative QTL regions for marbling: three on BTA6, two on BTA17, two on BTA22, and two on BTA29. We detected candidate genes for marbling within 1 cM of either side of the putative QTL regions. Additionally, to understand the functions of these candidate genes at the molecular level, we conducted a functional categorization using gene ontology and pathway analyses for those genes involved in lipid metabolism or fat deposition. In these putative QTL regions, we found 95 candidate genes for marbling. Using these candidate genes, we found five genes that had a direct interaction with the candidate genes. We also found SCARB1 as a putative candidate gene for marbling that involves fat deposition related to cholesterol transport.
摘要译文
肌肉脂肪的大理石花纹是肉质的重要特征，对牛肉产业具有经济效益。使用10K单核苷酸多态性组和组合连锁和连锁不平衡方法进行t loci（QTL）精细定位以鉴定266个Hanwoo阉牛的大理石花纹性状。结果，我们发现了九个用于大理石花纹的QTL区域：三个在BTA6上，两个在BTA17上，两个在BTA22上，两个在BTA29上。我们在假定的QTL区域的任一侧的1cM内检测到用于大理石花纹的候选基因。另外，要在分子水平上理解这些候选基因的功能，我们使用基因本体和通路分析对涉及脂质代谢或脂肪沉积的那些基因进行了功能分类。在这些推定的QTL区域，我们发现了95个用于大理石花纹的候选基因。使用这些候选基因，我们发现了五个与候选基因直接相互作用的基因。我们还发现SCARB1是大理石花纹的推定候选基因，涉及与胆固醇转运相关的脂肪沉积。
Park, Hye-Sun; Seo, Seong-Won; Cho, Yong-Min; Oh, Sung-Jong; Seong, Hwan-Hoo; Lee, Seung-Hwan; Lim, Da-Jeong;. Identification of Candidate Genes Associated with Beef Marbling Using QTL and Pathway Analysis in Hanwoo (Korean Cattle)[J]. Asian-Australasian Journal of Animal Sciences, 2012,25(5): 613-620