期刊文献

Cell-free synthesis of bacteriophage T4 glucosyl transferase 收藏

无细胞合成噬菌体T4葡萄糖基转移酶

原文求助发布源

作者

Elton T.YoungII[†];

作者单位

Institut de Biologie Moléculaire, Université, Geneva, Switzerland[†]Present address: Departments of Biochemistry and of Genetics, University of Washington, Seattle, Washington, 98105 U.S.A.

发布日期

14 August 1970

页码

591-604

DOI

10.1016/0022-2836(70)90010-0

来源信息

Journal of Molecular Biology ISSN：0022-2836, 1970年, 51卷, 3期, 591-604页

摘要

Either messenger RNA from infected cells or T4 DNA itself directs the cell-free synthesis of phage glucosyl transferase. The appearance of enzymic activity in vitro depends on protein synthesis and also on RNA synthesis in the coupled system. A product of the reaction catalyzed by the in vitro-synthesized transferase has been identified as α-glucosylated hydroxymethylcytosine, demonstrating the de novo synthesis of α-glucosyl transferase in the cell-free system described.

摘要译文

来自感染细胞的信使RNA或T4 DNA本身指导噬菌体葡糖基转移酶的无细胞合成。体外酶活性的出现取决于蛋白质合成以及偶联系统中的RNA合成。由体外合成的转移酶催化的反应产物已被鉴定为α-葡糖基化的羟甲基胞嘧啶，证明了在所述无细胞系统中α-葡萄糖基转移酶的从头合成。

Elton T.YoungII[†];. Cell-free synthesis of bacteriophage T4 glucosyl transferase[J]. Journal of Molecular Biology, 1970,51(3): 591-604