摘要
In an aqueous buffer system, serious reverse and side reactions were found in the asymmetric reduction of 2-octanone with Saccharomyces cerevisiae. However, some nonionic surfactants added to the aqueous buffer system improved the bioreduction process by decreasing the reverse and side reaction rates in addition to effectively increasing the average positive reaction rate. Further, a shorter carbon chain length of hydrophilic or hydrophobic moieties in surfactants resulted in a higher yield of (S)-2-octanol. The alkylphenol ethoxylate surfactants had a less influence than polyoxyethylenesorbitan trialiphatic surfactants on the product e.e. It suggested that the product e.e. resulting from the change of carbon chain length of the hydrophobic moieties varied markedly compared with the change of carbon chain length of the hydrophilic moiety. Emulsifier OP-10 and Tween 20 markedly enhanced the yield and product e.e. at the concentration of 0.4 mmol L−1 with a yield of 73.3 and 93.2%, and the product e.e. of 99.2 and 99.3%, respectively, at the reaction time of 96 h.
摘要译文
在含水缓冲系统中，在用酿酒酵母（Saccharomyces cerevisiae）不对称还原2-辛酮中发现了严重的反向和副反应。然而，除了有效地提高平均阳性反应速率之外，添加到水性缓冲系统中的因子通过降低反向和副反应速率改善了生物还原过程。此外，表面活性剂中亲水或疏水部分的碳链长度较短导致（S）-2-辛醇的产率较高。烷基酚乙氧基化物表面活性剂对产物e.e.的影响小于聚氧乙烯山梨糖醇酯脂族表面活性剂。它建议产品e.e.与亲水部分的碳链长度的变化相比，由疏水部分的碳链长度的变化引起的变化显着地变化。乳化剂OP-10和吐温20显着提高了产率和产物e.e.浓度为0.4 mmol L sup -1 / sup，产率为73.3和93.2％，产物e.e. 99.2％和99.3％，分别在96小时的反应时间。
Yunquan Zheng[1][4]]Liangbin Li[1]]Xianai Shi[2][4]]Zhijian Huang[3][4]]Feng Li[4]]Jianmin Yang[4]]Yanghao Guo[4]]. Nonionic surfactants and their effects on asymmetric reduction of 2-octanone with Saccharomyces cerevisiae[J]. AMB Express, 2018,8(1)