摘要
The objective of this study was to test the efficacy of different Propionibacterium strains in mitigating methane (CH4) emissions in beef heifers fed a high-forage diet. Twenty ruminally cannulated beef heifers were used in a randomized block design with 28-d periods. Treatments included 1) Control, 2) Propionibacterium acidipropionici strain P169, 3) Propionibacterium acidipropionici strain P5, and 4) Propionibacterium jensenii strain P54. Strains (5 × 109 CFU) were administered daily directly into the rumen in 10 g of a maltodextrin carrier in a gel capsule. Control heifers received the carrier only. All heifers were fed a basal diet (70:30 forage to concentrate, DM basis) based on barley silage and corn grain. No treatment effects were observed for overall DMI (P = 0.78) or DMI in chambers (P = 0.29). Dry matter intake was 12 to 29% less in the chambers, with intake depression numerically lower in heifers receiving Propionibacterium than Control. Mean ruminal pH averaged 6.47 and was not affected by treatments (P = 0.34). Likewise, no treatment differences were observed for ruminal concentrations of total VFA (P = 0.24) and ammonia-N (P = 0.49) or for molar proportion of individual VFA. Total daily enteric CH4 production was not affected by Propionibacterium strains as compared to Control and averaged 178 g/d (P = 0.69). However, enteric CH4 emission intensity (g CH4/kg of DMI) was reduced by 12, 8, and 13% with P169, P5, and P54 as compared to Control, respectively (P = 0.03). No treatment effects were observed for total tract digestibility of nutrients. Likewise, total universal bacterial (P = 0.22) and methanogen (P = 0.64) counts were similar among treatments. However, the relative abundance of total Propionibacteria tended to increase with inoculation as compared to Control (P = 0.06). The relative abundance of Propionibacterium P169 tended to be greater at 3 h postdosing, but returned to pretreatment (0 h) levels within 9 h, suggesting it failed to persist at detectable levels in the rumen. In conclusion, Propionibacterium spp. did not reduce total enteric CH4 production, possibly due to their inability to persist and integrate into the ruminal microbial community. However, CH4 emission intensity was reduced with Propionibacterium strains, a response attributed to the numerically greater DMI of heifers receiving Propionibacterium.
摘要译文
这项研究的目的是为了测试不同丙酸杆菌菌株在缓解高饲料日粮牛肉小牛甲烷（CH 4）排放方面的功效。在随机区组设计中使用20个瘤胃插管的牛小母牛，具有28天的时间段。处理包括1）对照，2）丙酸丙酸杆菌菌株P169，3）产丙酸丙酸杆菌菌株P5，和4）简氏丙酸杆菌菌株P54。将菌株（5×10 9 CFU）每日直接施用于10g麦芽糖糊精载体中的凝胶胶囊中的瘤胃中。控制小母牛只收到运营商。基于大麦青贮饲料和玉米粒，所有小母牛都进食基础日粮（70:30饲料浓缩，DM基础）。整体DMI没有观察到治疗效果（P \x3d 0。78）或室内DMI（P \x3d 0.29）。室内干物质摄入量减少12％至29％，接受丙酸杆菌的小母牛的摄入量在数值上低于对照。平均瘤胃pH值平均为6.47，不受治疗影响（P \x3d 0.34）。同样，对于总VFA（P \x3d 0.24）和氨-N（P \x3d 0）的瘤胃浓度没有观察到处理差异。49）或个体VFA的摩尔比例。与对照相比，丙酸杆菌菌株没有影响每日总肠溶CH 4产量，平均为178g / d（P \x3d 0.69）。然而，与对照相比，P169，P5和P54的肠道CH 4排放强度（g CH 4 / kg DMI）分别降低了12％，8％和13％（P \x3d 0.03）。对营养物质的全消化道消化率没有观察到治疗效果。同样，治疗中总通用细菌（P \x3d 0.22）和产甲烷菌（P \x3d 0.64）计数相似。然而，与对照相比，接种后总丙酸杆菌的相对丰度趋于增加（P \x3d 0.06）。丙酸杆菌P169的相对丰度在给药后3小时倾向于更大，但在9小时内恢复到预处理（0小时）水平，暗示它没有在瘤胃中持续检测到的水平。总之，Propionibacterium spp。没有减少肠道CH 4总产量，可能是由于它们无法坚持并融入瘤胃微生物群落。然而，用丙酸杆菌菌株降低CH 4排放强度，归因于接受Propionibacterium的小母牛在数量上更大的DMI的响应。
D. Vyas;E. J. McGeough;S. M. McGinn;T. A. McAllister;K. A. Beauchemin. Effect of Propionibacterium spp. on ruminal fermentation, nutrient digestibility, and methane emissions in beef heifers fed a high-forage diet[J]. Journal of Animal Science, 2014,92(5): 2192–2201