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The First Report on Chromosome Analysis of F1 Hybrid Catfish: Mekong Giant Catfish (Pangasianodon gigas)×Striped Catfish (Pangasianodon hypophthalmus) and Spot Pangasius (Pangasius larnaudii)×Pangasianodon hypophthalmus (Siluriformes, Pangasiidae) 收藏

关于F 1杂交鲶鱼染色体分析的第一个报告：湄公河巨型鲶鱼（Pangasianodon Gigas）×条纹鲶鱼（Pangasianodon次骨斜肌）和点Pangasius（Pangasius Larnaudii）×Pangasianodon次斜滴症（SiluRiformes，Pangasiidae）
原文求助 发布源
作者
Kosit Sreeputhorn[1];Kriangsak Mangumphan[2];Benjawon Muanphet[2];Alongklod Tanomtong[3];Weerayuth Supiwong[4];Puntivar Kaewmad[5]
作者单位
[1]Faculty of Natural Resources, Rajamangala University of Technology Isan, Sakon Nakhon Campus;[2]Faculty of Fisheries Technology and Aquatic Resources, Maejo University;[3]Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University;[4]Faculty of Applied Science and Engineering, Khon Kaen University;[5]Major of Biology, Faculty of Science and Technology, Mahasarakham Rajabhat University
页码
457-463
DOI
10.1508/cytologia.82.457
来源信息
CYTOLOGIA ISSN：0011-4545, 2017年, 82卷, 4期, 457-463页
摘要
The first chromosomal characteristic of F1 hybrid catfish from Thailand was studied. Kidney cell samples were taken from four male and four female fish. The mitotic chromosome preparations were done directly from kidney cells. Conventional staining technique was applied to stain the chromosomes. The results showed that the diploid chromosome number of Mekong giant catfish (Pangasianodon gigas)×striped catfish (P. hypophthalmus) and spot pangasius (Pangasius larnaudii)×P. hypophthalmus were 2n=60, the fundamental number (NF) was 108 in both males and females. The chromosomes presences of metacentric, submetacentric, acrocentric, and telocentric chromosomes were 27-11-10-12 and 25-12-11-12, respectively. While the chromosomes of P. gigas, P. hypophthalmus, and P. larnaudii (parents) presences of metacentric, submetacentric, acrocentric, and telocentric chromosomes were 28-12-12-8, 26-10-8-16, and 24-14-14-8, respectively. Results obtained will increase our basic knowledge of the cytogenetics of the family Pangasiidae which could form the basis for future research and provide data to ensure their survival. The karyotype formulae could be deduced as:
摘要译文
研究了泰国F 1杂种鲶鱼的第一个染色体特征。肾细胞样品取自四只雄鱼和四只雌鱼。有丝分裂染色体制备直接从肾细胞进行。应用常规染色技术染色染色体。结果表明，湄公河巨鲶（Pangasianodon gigas）×条纹鲶鱼（P. hypophthalmus）和斑鲶（Pangasius larnaudii）×P的二倍体染色体数目。眼球突出2n \x3d 60，男性和女性的基本数（NF）均为108。染色体存在中心，亚中心，acrocentric，和中心染色体分别为27-11-10-12和25-12-11-12。而P. gigas，P。hypophthalmus和P. larnaudii（父母）的染色体存在于中心，亚中心，中心和中心染色体分别为28-12-12-8,26-10-8-16和24-14-14-8。获得的结果将增加我们对Pangasiidae家族细胞遗传学的基本知识，这可能成为未来研究的基础，并提供数据以确保其存活。核型公式可以推导为：
Kosit Sreeputhorn[1];Kriangsak Mangumphan[2];Benjawon Muanphet[2];Alongklod Tanomtong[3];Weerayuth Supiwong[4];Puntivar Kaewmad[5]. The First Report on Chromosome Analysis of F1 Hybrid Catfish: Mekong Giant Catfish (Pangasianodon gigas)×Striped Catfish (Pangasianodon hypophthalmus) and Spot Pangasius (Pangasius larnaudii)×Pangasianodon hypophthalmus (Siluriformes, Pangasiidae)[J]. CYTOLOGIA, 2017,82(4): 457-463