摘要
To better understand the prevalence of Gallibacterium anatis in different poultry species, a rapid and accurate method was developed to detect G. anatis using a TaqMan fluorescent quantitative polymerase chain reaction (qPCR). Specific primers and a TaqMan probe were designed based on the reference gtxA gene sequence. The qPCR standard curve showed a good linear relationship, and the method showed good reproducibility, sensitivity, and specificity, indicating its suitability for G. anatis identification and quantitative analysis. A comparison of the detection results in 160 clinical swab samples showed that the detection rate (54.4%) of the qPCR for G. anatis was better than that of two conventional methods: gyrB gene-based qPCR for G. anatis (51.9%) and culture-based identification (34.4%). G. anatis was detected in layer chicken (77.3%), Silkie chicken (72.7%), and duck (27.1%) with relatively high detection rates, whereas dove (8.8%) and quail (3.0%) showed lower detection rates, indicating the different prevalence of G. anatis in different fowl species.
摘要译文
为了更好地了解不同家禽品种中杆状杆菌的流行情况，我们开发了一种快速准确的方法来检测G.使用TaqMan荧光定量聚合酶链式反应（qPCR）进行解剖。基于参考gtxA基因序列设计特异性引物和TaqMan探针。qPCR标准曲线显示出良好的线性关系，该方法显示出良好的重现性，灵敏度和特异性，表明其适用于G.解剖学鉴定和定量分析。对160份临床拭子样本的检测结果进行比较，结果显示G的qPCR检出率（54.4％）。anatis优于两种常规方法：基于gyrB基因的针对G. anatis的qPCR（51.9％）和基于培养的鉴定（34.4％）。在层鸡中检测到G. anatis（77.3％），Silkie鸡（72.7％）和鸭（27.1％）的检出率较高，而鸽子（8.8％）和鹌鹑（3.0％）的检出率较低，表明G的不同流行率。在不同的禽类物种的anatis。
Heping Huangfu[1];Wenbo Xu[2];Hongkui Wang[2];Qing Dong[2];Hongwei Guo[2];Yanting Sun[2];Yunxia Li[2];Wenwen Gao[2];Wuqing Wang[2];Jia Zhang[2];Jingke Shi[2];Haochun Pan[2];Congcong Li[2];Linkang Wang[2]. Detection of Gallibacterium anatis by TaqMan fluorescent quantitative PCR[J]. Avian Pathology, 2018,47(3): 245-252