摘要
The present study was conducted to examine the effects of culture systems and culture media on developmental competence and freezability of bovine embryos obtained by in vitro culture of in vitro matured and fertilized (IVM-IVF) oocytes. No significant difference was observed in the proportions of oocytes developed to blastocysts, the speed at which the oocytes reached the blastocyst stage and the number of cells, when the IVM-IVF oocytes were cultured in CR1aa with or without cumulus cells. Nevertheless, more of the IVM-IVF oocytes cultured either with or without cumulus cells in CR1aa were seen to reach the blastocyst stage much sooner than those cultured with cumulus cells in TCM199 (P<0.05). The proportion of embryos developed to the blastocyst stage by day 7 in CR1aa culture was significantly higher than embryos cultured in TCM199. Viability after frozen-thawed blastocysts were obtained in vitro, was seen in a significantly higher percentage of embryos cultured in TCM199 and developed to the hatched blastocysts than in those cultured in CR1aa (P<0.05). These results indicate that CR1aa was superior to TCM199 for the potential developmental of IVM-IVF oocytes to blastocysts during in vitro culture regardless of co-culture with or without cumulus cells. But the freezability of blastocysts developed in CR1aa was inferior to those developed in TCM199.
摘要译文
xamine培养系统和培养基上发育能力和通过体外培养的体外成熟，受精（IVM-IVF）的卵母细胞得到的牛胚胎freezability的影响。无显著差异，观察在卵母细胞的发展到囊胚的比例，速度在该卵母细胞达到胚泡期和细胞的数量，当在IVM-IVF卵母细胞培养在CR1aa有或无卵丘细胞。然而，更在IVM-IVF卵母细胞具有或不卵丘细胞中CR1aa中培养的，被视为比具有卵丘细胞培养在TCM199更快达到胚泡期（P 0.05）。在CR1aa培养发展到胚泡期7天的胚胎的比例比胚在TCM199培养显著高。在体外获得冻融囊胚后生存能力，被认为在胚胎的显著更高的百分比在TCM199培养并发展到孵化囊胚比那些培养CR1aa（P 0.05）。HESE结果表明CR1aa优于TCM199为IVM-IVF卵母细胞的胚泡体外培养过程中的潜在发育无论共培养的有或无卵丘细胞。但在CR1aa开发囊胚的freezability不如那些TCM199发展。
Kei IMAI[1]; Satoko MATOBA[2]; Osamu DOCHI[3];Itsuo SHIMOHIRA[2]. Different Factors Affect Developmental Competence and Cryotolerance in in vitro Produced Bovine Embryo[J]. Journal of Veterinary Medical Science, 2002,64(10): 887-891