摘要
Testosterone (T) and 5α-dihydrotestosterone (17β-hydroxy-5α-androstan-3-one; DHT) are bound to specific cytoplasmic receptors (CR) in 105, 000 × g supernatant fractions of seminiferous tubules from hypophysectomized rats following the intravenous injection of [1, 2-3h]testosterone. CR is clearly different from the testicular androgen binding protein (ABP) by electrophoretic mobility, temperature stability and rate of dissociation of steroid-CR complex, but very similar to the cytoplasmic receptors of epididymis and ventral prostate. Under these labeling conditions, the nuclei of seminiferous tubules also contain radioactive T and DHT bound to protein. These androgen-protein complexes, which can be extracted with 0.4 M − 1 M KC1, have a sedimentation coefficient of 3–4 S. Binding of radioactive T and DHT to both cytoplasmic and nuclear receptors in vivo is specific for androgen target tissues and abolished by simultaneous injection of unlabeled T, DHT and cyproterone acetate (1, 2-α-methylene-6-chloro-pregn-4, 6-diene-17α-o1–3, 20-diene-17-acetate), but not by cortisol. It is suggested that receptors for testosterone and DHT in the seminiferous tubules are involved in the mediation of the androgenic stimulus to the germ cells.
摘要译文
睾酮（T）和5α-二氢睾酮（17β-羟基-5α-雄甾-3-酮; DHT）与特异性细胞质受体（CR）结合在105，静脉内注射[1,2- sup 3 / sup h]睾酮后，来自垂体切除大鼠的曲细精管的000×g上清液部分。通过电泳迁移率，温度稳定性和类固醇-CR复合物的解离速率，CR明显不同于睾丸雄激素结合蛋白（ABP），但非常类似于附睾和腹侧前列腺的细胞质受体。在这些标签条件下，曲细精管的细胞核还含有与蛋白质结合的放射性T和DHT。这些雄激素 - 蛋白质复合物，可以用0.4 M - 1 M KC1提取，沉降系数为3-4 S.放射性T和DHT在体内对细胞质和核受体的结合对雄激素靶组织是特异性的，并且通过同时注射未标记的T而消除，DHT和醋酸环丙孕酮（1,2-α-亚甲基-6-氯 - 孕-4,6-二烯-17α-o1-3,20-二烯-17-乙酸酯），但不是皮质醇。有人提出，曲细精管中的睾酮和DHT受体参与雄激素刺激生殖细胞的介导。
V. Hansson A.A. Smith D.J. Tindall S.C. Weddington S.N. Nayfeh F.S. French. Androgen receptors in rat testis[J]. Steroids, 1974,23(5): 823-832