摘要
Controlled laboratory culture of Alexandrium catenella was used to determine the effects of a range of temperatures between 10 and 16 °C on the growth and saxitoxin content of this dinoflagellate, using strain ACC02 isolated from seawater at Aysen, XI Region, Southern Chile. Cell cultures were made using L1 culture medium at 30‰ salinity, and a photon flux density of 59.53 μmol m2 s−1. The results showed that the duration of the exponential growth phase was determined by the experimental temperature, with maximum cell concentrations obtained at 12 °C; significantly lower cell concentrations and growth rates were obtained at 16 °C. Cell dry weight and chlorophyll a values followed cell growth trends. The toxicity of A. catenella was variable at all the experimental temperatures, with a tendency towards having an inverse relation to temperature, with the highest values occurring at 10 °C and the lowest at 16 °C. The optimal range of temperature for the growth of the Chilean strain of A. catenella differed from rates reported for this species isolated at other latitudes, and was correlated with natural temperature conditions predominant in the environment from which it was isolated. The inverse relation of toxicity with temperature in the laboratory was broadly reflected in observations on the toxicity of this dinoflagellate in the field, and coincided with results from the literature.
摘要译文
亚历山大藻链霉菌的受控实验室培养用于确定10至16℃范围内的温度对该甲藻的生长和蛤蚌毒素含量的影响，使用从智利南部XI区Aysen的海水中分离的菌株ACC02。使用浓度为30‰的L1培养基制备细胞培养物，光子通量密度为59。53μmolmsup 2 / sup s sup -1 / sup。结果表明，指数生长期的持续时间由实验温度决定，在12°C时获得最大细胞浓度;在16℃下获得显着更低的细胞浓度和生长速率。细胞干重和叶绿素a值遵循细胞生长趋势。在所有实验温度下，A。catenella的毒性是变化的，倾向于与温度成反比关系，最高值出现在10°C，最低值出现在16°C。智利A. catenella菌株生长的最佳温度范围与在其他纬度分离的该物种的报告率不同，并且与在其分离的环境中占主导地位的自然温度条件相关。实验室中毒性与温度的反比关系广泛地反映在该鞭毛藻在田间的毒性观察中，并与文献中的结果相吻合。
J.M.Navarro;M.G.Muñoz;A.M.Contreras;. Temperature as a factor regulating growth and toxin content in the dinoflagellate Alexandrium catenella[J]. Harmful Algae, 2006,5(6): 762-769