摘要
Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.
摘要译文
从植物相关的发酵食品和作物中分离植物乳酸菌，并研究它们的益生菌性质。从泡菜和洋葱中分离出分离株K27和O2，并在16S rRNA基因分析的基础上鉴定为植物乳杆菌。这两种菌株对酸具有高度抗性（pH值为2.5的MRS肉汤），其中植物乳杆菌K27和植物乳杆菌O2的存活率分别为90.2％和97.3％。植物乳杆菌K27和植物乳杆菌O2也显示出对0.5％oxgall的高胆汁抗性，存活率超过70％。它们显示出对大肠杆菌KCCM 40880和铜绿假单胞菌ATCC 10145的致病菌株的抑制作用。两种菌株的抗菌作用可能是由于乳酸的存在。在MRS肉汤中，两种菌株的ACE抑制活性范围为72.8％至80.6％。值得注意的是，这两种菌株在含有10％脱脂乳的MRS肉汤中显示出高ACE抑制活性（89.2~98.2％）。通过DPPH自由基清除活性测试抗氧化活性，菌株的抗氧化活性在56.8％~61.5％的范围内。在该研究中获得的结果表明植物乳杆菌K27和植物乳杆菌O2可能是具有发酵产物应用的潜在益生菌起子培养物。
Kim, Jeong-Do; Park, Sung-Bo; Lee, Na-Ri; Jeong, Jin-Ha; Lee, Hee-Seob; Hwang, Dae-Youn; Lee, Jong-Sup; Jeong, Seong-Yun; Son, Hong-Joo;. Isolation and Characterization of Plant-Derived Lactic Acid Bacteria as Potential Probiotic[J]. Microbiology and Biotechnology Letters, 2011,39(3): 308-312