摘要
The intrauterine inseminator (IUI) was developed to provide the method of depositing dog semen into the uterine body instead of the vagina. The IUI consists of a vaginal endoscope, a balloon sheath, and injection catheter. When the endoscope is inserted into the vagina and the balloon expanded with air, the cervical os becomes visible so a injection catheter can be inserted through the cervix for deposition of the frozen-thawed semen. The efficacy of the IUI device was compared to intra-vaginal artificial insemination using semen that had been collected and frozen from pooled sperm-rich fraction of ejaculates collected from two Jindo dog donors. Aliquots of semen were extended with a Tris-egg yolk diluent, centrifuged, the seminal plasma removed, the pellet resuspended with the same diluent, and cooled to over a 2 h period. A Tris-egg yolk-glycerol extender was added at ; after 1 h, semen was loaded into 0.5 ml straws, and straws were frozen in LN vapor for 5 min, and immersed in LN for storage. The final sperm concentration for freezing was approximately . The straws were thawed at for precisely 6 sec, 1.5 ml Tris-egg yolk buffer at added, and the 2 ml of thawed semen was used for a single insemination using the IUI device. Each bitch was inseminated at optimal insemination point, which was estimated by vaginal epithelial cells staining and progesterone concentration analysis. Use of the IUI device resulted in 21 of 26 females giving birth to 89 pups ( pups per litter), while intra-vaginal AI resulted in 6 of 15 females whelping a total of 17 pups ( pups per litter). We believe the IUI device is easier to use than previously described devices used for intrauterine insemination. In our experience the expansion of the balloon has a calming effect on the bitch that aids the inseminator. These results indicate that the IUI device was able to provide high fertility with 50 million frozen sperm per insemination and two inseminations.
摘要译文
开发了子宫内授精器（IUI）以提供将狗精液沉积到子宫体而不是阴道中的方法。 IUI由阴道内窥镜，气囊鞘，和注射导管。当内窥镜插入阴道并且气球随空气膨胀时，宫颈口变得可见，因此可以通过子宫颈插入注射导管以沉积冷冻 - 解冻的精液。将IUI装置与使用精液的阴道内人工授精进行比较，所述精液已经从两个Jindo狗供体收集的精子富集的精液部分中收集和冷冻。将等分的精液用Tris-蛋黄稀释液延长，离心，除去精浆，用相同的稀释剂重悬沉淀，并冷却至2小时。加入Tris-蛋黄 - 甘油增量剂; 1小时后，将精液加入0.5ml吸管中，将吸管在LN蒸气中冷冻5分钟，并浸入LN中储存。冷冻的最终精子浓度约为。将吸管解冻6秒钟，加入1.5ml Tris-egg蛋黄缓冲液，并且使用IUI装置将2ml解冻的精液用于单次授精。每个母狗在最佳授精点进行授精，通过阴道上皮细胞染色和孕酮浓度分析估计。使用IUI装置导致26名女性中的21名生下89只幼仔（每窝幼仔），阴道内AI导致15只雌性中有6只幼仔共有17只幼仔（每窝幼仔）。我们相信IUI设备比先前描述的用于子宫内人工授精的设备更容易使用。根据我们的经验，气球的膨胀对母狗产生了镇静作用，有助于授精。这些结果表明IUI装置能够提供高生育力，每次授精和两次授精，每年有5000万个冷冻精子。
Kong, I.K.; Yu, D.J.; Jeong, S.R.; Oh, I.S.; Yang, C.J.; Cho, S.G.; Bae, I.H.; Oh, D.H.; Kim, H.R.; Cho, S.K.; Park, C.S.;. A New Device for Intrauterine Artificial Insemination in the Dog[J]. Asian-Australasian Journal of Animal Sciences, 2003,16(2): 180-184