摘要
Lipid content and composition of some dominant copepods inhabiting the Oyashio waters were analyzed by the thin layer chromatography flame ionization detection (TLC/FID) method. Copepod samples were collected during the RV Shoyo-maru cruise from September 18 to October 15 in 1988. A new three-step development: the first in hexane and diethyl ether (66 : 4, v/v), the second in hexane, diethyl ether and formic-acid (55 : 15 : 0.5) and the last in hexane and benzene (1 : 1), single scan method by TLC/FID was found to be useful for separation and analysis of lipids in boreal copepods. As a result, the eight species of calanoid copepods analyzed in this study could be divided into two groups according to their lipid class proportions. One group consisted of copepods containing many wax esters and includes Neocalanus flemingeri, N. cristatus, Pseudocalanus minutus, Metridia pacifica and M. okhotensis. The other group containing many triglycerides includes Eucalanus bungii, Pleuromamma abdominalis and Acartia omorii. I have deduced that copepods storing wax esters could have long periods of dormancy in winter and consume lipids mainly for reproduction, while copepods storing triglycerides could overwinter without a period of dormancy and be opportunistic feeders.
摘要译文
一些优势桡足类居住的亲潮水域的脂质含量和组合物是由薄层色谱火焰电离检测（TLC / FID）方法进行分析。首先在正己烷和乙醚：在RV逍遥丸邮轮从9月18日至10月15日在1988年一个新的三步走发展过程中所收集的样品桡足类（66：4，V / V），第二次在己烷，乙醚和甲酸 - 乙酸（55：15：0.5）和最后在己烷和苯（1：1），用TLC / FID单一扫描方法被认为是用于在寒带桡足类脂质的分离分析是有用的。其结果，的8种在这项研究中分析了哲水蚤桡足类可以根据自己的类脂质的比例被分成两组。一组由包含许多蜡酯桡足类，包括Neocalanus flemingeri，N.狗尾草，Pseudocalanus蝽，Metridia的Pacifica和M. okhotensis。含有多种甘油三酯，另一组包括Eucalanus天牛，Pleuromamma腹和纺锤omorii。我推断，桡足类存储蜡酯可能在冬季休眠长时间，消耗脂肪主要用于再生产，而桡足类存储甘油三酯可以不越冬休眠期，并伺机馈线。
Yuichi Kotani[1]. Lipid content and composition of dominant copepods in the Oyashio waters analyzed by the thin layer chromatography flame ionization detection method[J]. Plankton and Benthos Research, 2006,1(2): 85-90