摘要
Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0–2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.
摘要译文
最近在狗中检测到了沙波病毒属的杯状病毒。在患有胃肠疾病的青少年动物的粪便中（2.0-2.2％），已经鉴定了犬唾液病毒（SaV），但它们是否可能具有作为肠道病原体的作用，以及它们在何种程度上暴露于SaV还不清楚。在这里，我们报道了犬SaV毒株的病毒样颗粒（VLPs）的杆状病毒系统中的表达，原型病毒Bari / 4076/2007 / ITA。重组抗原用于开发酶联免疫吸附测定（ELISA）。通过筛选来自意大利的516只狗的年龄分层的血清样品收集，在40.3％（208/516）的血清中检测到针对犬SaV VLP特异性的IgG抗体。此外，正如在人类中观察到的SaV感染，我们观察到血清阳性与年龄之间的正相关，在4岁以上的狗中患病率最高。
IreneMelegari[a];FulvioMarsilio[a];FedericaDi Profio[a];VittorioSarchese[a];IvanoMassirio[b];AndreaPalombieri[a];Anna RitaD’Angelo[c];GianvitoLanave[d];GeorgiaDiakoudi[d];AlessandraCavalli[d];VitoMartella[d];BarbaraDi Martino[a];. Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles[J]. Virus Research, 2018,251: 1-5