摘要
. Cooled canine semen solutions for storage were investigated with three stock solutions: egg yolk-citrate-glycine-glucose solution, egg yolk Tris-fructose citrate solution (EYT-FC), and egg yolk sodium citrate dihydrate solution (EYCD). For the control group, the second fraction of semen was examined. Nine male beagles and 37 female (47 experimental cases) beagles for artificial insemination (AI) were used. The qualities of semen stored at 4°C deteriorated earlier in the control and EYCD groups. In the other two groups, sperm motility was 60% or higher after storage for 6 days and 20% or higher after storage for 12 days. On a comparison of these two groups, the sperm motility and viability were slightly higher in the EYT-FC group. A high conception rate was obtained by AI using semen stored at a low temperature for a maximum of two days in the control group and four days in the EYT-FC group.
摘要译文
。存储冷却犬精液的解决方案进行了研究有三个股票的解决方案：蛋黄柠檬酸甘氨酸葡萄糖液，蛋黄的Tris-果糖柠檬酸溶液（EYT-FC），和蛋黄二水柠檬酸钠溶液（EYCD）。为对照组，精液的第二级分进行了检查。九阳小猎犬和37女（47实验例）比格犬人工授精（AI）的使用。保存在4℃的精液质量恶化早些时候在控制和EYCD组。在其它两组，精子活力为60％或贮存12天后贮存6天和20％后更高或更高。关于这两组的比较中，精子活力和存活率分别为所述EYT-FC组中略高。高受孕率，使用储存在低温下一个最大的，对照组2天，4天EYT-FC组精液中由人工智能获得。
Toshihiko TSUTSUI[1]; Tomoyuki TEZUKA[1]; Yukiko MIKASA[1]; Hirofumi SUGISAWA[1]; Nobuyuki KIRIHARA[1]; Tatsuya HORI[1];Eiichi KAWAKAMI[1]. Artificial Insemination with Canine Semen Stored at a Low Temperature[J]. Journal of Veterinary Medical Science, 2003,65(3): 307-312