摘要
Forest musk deer (FMD) is an endangered species, and its population size has dropped dramatically. In this study, we determined the complete mitochondrial genome of a FMD. The genome was 16,353 bp long, and contained 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes, and one control region. Most PCGs were distributed on the heavy strand except ND6 and eight tRNA genes, which were encoded on the light strand. The nucleotide composition was asymmetric, with an overall A+T content of 62.1%. Comparison with one previously described mitochondrial genome revealed 94.4% sequence homology and 1128 nucleotide mutation sites, which represents a substantial difference. A phylogenetic tree based on Cytb genes of eight closely related musk deer species showed that our sample clustered with two FMD subspecies from Yunnan, China. These results provide novel molecular information that can potentially be used for genetic diversity conservation of this species.
摘要译文
森林麝（FMD）是一种濒临灭绝的物种，其种群规模急剧下降。在这项研究中，我们确定了FMD的完整线粒体基因组。该基因组长16,353bp，含有13个蛋白质编码基因（PCGs），22个转移RNA（tRNA）基因，2个核糖体RNA基因和1个对照区域。大多数PCG分布在重链上，除了ND6和8个tRNA基因，它们编码在轻链上。核苷酸组成是不对称的，总A + T含量为62.1％。与先前描述的线粒体基因组的比较显示94.4％的序列同源性和1128个核苷酸突变位点，这代表显着差异。基于八种密切相关的麝鹿种的Cytb基因的系统发育树显示，我们的样本聚集了来自中国云南的两个FMD亚种。这些结果提供了新的分子信息，可以用于该物种的遗传多样性保护。
Qian Su[1]]Yongfang Yao[1]]Diyan Li[2]]Huaming Xu[1]]Jiayun Wu[1]]Anxiang Wen[1]]Meng Xie[1]]Qingyong Ni[2]]Mingwang Zhang[2]]Huailiang Xu[1]]. The complete mitochondrial genome sequence of a wild forest musk deer (Moschus berezovskii)[J]. Conservation Genetics Resources, 2018,10(3): 329-333