摘要
Two in vitro incubations utilizing batch rumen culture procedures were conducted to study the effects of Monensin and Tween 80, alone and in combination, on ruminal fermentation. Dried, ground backgrounding and finishing diets for feedlot cattle were used as substrates in experiments 1 and 2, respectively. The diets, formulated as total mixed rations, contained (DM basis) barley grain and barley silage in ratios of 58:42 (backgrounding diet, BKGD) or 93:7 (finishing diet, FNSH). Inoculum comprised ruminal fluid collected from cattle fed BKGD or FNSH diluted with two volumes of mineral buffer, and amended with Monensin (0, 2.5 or 15 μg/ml) and Tween 80 (0, 0.1 or 0.5 μl/ml) in a 3 × 3 factorial arrangement of treatments. Incubations were conducted anaerobically for 4, 12 or 24 h at 39 °C (n = 3). 15N-labelled ammonium sulphate was included to enable measurement of microbial N production (MN). Interactive effects (P h of incubation, on MN at 4 and 24 h, and on accumulation of reducing sugars (RS) at 4, 12 and 24 h were observed with BKGD, but not with FNSH. Tween 80 applied at 0.5 μl/ml increased (P h and MN at 24 h, but reduced (P h of incubation with BKGD. Similar effects of Tween 80 on RS and A:P ratio were observed with FNSH. In contrast, throughout 24 h of incubation with either substrate, Monensin reduced gas production (GP), TDMD, MN and the A:P ratio, and increased RS accumulation (BKGD, P P P P P P P
摘要译文
使用批次瘤胃培养程序进行两次体外培养以研究单独和组合的莫能菌素和吐温80对瘤胃发酵的影响。干燥，饲养场牛的地面背景和育肥日粮分别用作实验1和2中的底物。饮食，配制成全混合口粮，含有（DM基础）大麦籽粒和大麦青贮饲料的比例为58:42（背景日粮，BKGD）或93：7（最终日粮，FNSH）。接种物包含从用两体积矿物质缓冲液稀释的喂养BKGD或FNSH的牛收集的瘤胃液，并用莫能菌素（0,2.5或15μg/ ml）和吐温80（0,0.1或0）进行修正。5×1 / ml）在3×3因子排列的处理中。孵育在39℃下厌氧进行4,12或24小时（n \x3d 3）。添加N标记的硫酸铵以测量微生物N产生（MN）。交互作用（培养时间为4小时和24小时的MN，用BKGD观察4,12和24小时还原糖（RS）的积累，但不用FNSH观察到。以0.5μl/ ml施用的吐温80增加（P h和MN在24小时，但是减少了（用BKGD孵育P h。用FNSH观察吐温80对RS和A：P比例的相似影响。相比之下，在任何一种底物孵育24小时后，莫能菌素减少了天然气产量（GP），TDMD，MN和A：P比例，并增加了RS积累（BKGD，P P P P P P P
Y.Wang;T.W.Alexander;T.A.McAllister;. In vitro effects of Monensin and Tween 80 on ruminal fermentation of barley grain:barley silage-based diets for beef cattle[J]. Animal Feed Science and Technology, 2004,116(3-4): 197-209