摘要
Background
Understanding the host impact on its symbiotic microbiota is important in redirecting the rumen microbiota and thus improving animal performance. The current study aimed to understand how rumen microbiota were altered and re-established after being emptied and receiving content from donor, thus to understand the impact of such process on rumen microbial fermentation and to explore the microbial phylotypes with higher manipulation potentials.Results
Individual animal had strong effect on the re-establishment of the bacterial community according to the observed profiles detected by both fingerprinting and pyrosequencing. Most of the bacterial profile recovery patterns and extents at genus level varied among steers; and each identified bacterial genus responded to transfaunation differently within each host. Coriobacteriaceae, Coprococcus, and Lactobacillus were found to be the most responsive and tunable genera by exchanging rumen content. Besides, the association of 18 bacterial phylotypes with host fermentation parameters suggest that these phylotypes should also be considered as the regulating targets in improving host feed efficiency. In addition, the archaeal community had different re-establishment patterns for each host as determined by fingerprint profiling: it was altered after receiving non-native microbiome in some animals, while it resumed its original status after the adaptation period in the other ones.Conclusions
The highly individualized microbial re-establishment process suggested the importance of considering host genetics, microbial functional genomics, and host fermentation/performance assessment when developing effective and selective microbial manipulation methods for improving animal feed efficiency.
摘要译文
背景了解宿主对其共生微生物群的影响对于重定向瘤胃微生物群并因此改善动物性能非常重要。目前的研究旨在了解瘤胃微生物群在被清空并从供体接收内容后如何被改变和重建，从而了解这种过程对瘤胃微生物发酵的影响，并探索具有较高操纵电位的微生物种系。结果根据指纹图谱和焦磷酸测序检测到的观察结果，个体动物对细菌群落的重建具有强烈的影响。大多数细菌形态恢复模式和属的水平在转向期间不同;并且每个鉴定的细菌属在每个宿主内以不同的方式对transfaunation作出反应。通过交换瘤胃含量，发现了Coriobacteriaceae，Coprococcus和Lactobacillus是最具响应性和可调节的属。除了，18种细菌种系与宿主发酵参数的关联表明这些种系型也应被视为提高寄主饲料效率的调节目标。此外，通过指纹图谱分析确定古菌群落对每个宿主有不同的重建模式：在一些动物接受非本地微生物组后，它被改变了，在适应期过后，它恢复了原来的状态。结论高度个性化的微生物重建过程提出了考虑宿主遗传学，微生物功能基因组学，当开发有效和选择性微生物操作方法以改善动物饲料效率时，进行发酵/性能评估。
Mi Zhou[1]]Yong-Jia Peng[2]]Yanhong Chen[1]]Christen M. Klinger[1]]Masahito Oba[1]]Jian-Xin Liu[2]]Le Luo Guan[1]]. Assessment of microbiome changes after rumen transfaunation: implications on improving feed efficiency in beef cattle[J]. Microbiome, 2018,6(1)