期刊文献

Mitochondrial DNA repair by photolyase 收藏

通过光裂解酶修复线粒体DNA

原文求助发布源

作者

AkiraYasui[1];HirohikoYajima[1];TakayasuKobayashi[1];Andre P.M.Eker[2];AtsushiOikawa[1];

作者单位

[a]Research Institute for Tuberculosis and Cancer, Tohoku University, 4-1 Seiryomachi, Aobaku, Sendai 980 Japan[b]Department of Cell Biology and Genetics, Erasmus University Rotterdam, Rotterdam The Netherlands[∗]Correspondence: Dr. A. Yasui, Research Institute for Tuberculosis and Cancer, Tohoku University, 4-1 Seiryomachi, Aobaku, Sendai 980 Japan.

关键词

Photoreactivation; Yeast; Mitochondrial DNA; Protein transport; Uracil-DNA-glycosylase;

关键词译文

光复活;酵母;线粒体DNA;蛋白质运输;尿嘧啶DNA糖基化酶;

发布日期

1 March 1992

页码

231-236

DOI

10.1016/0921-8777(92)90084-G

来源信息

Mutation Research/DNA Repair ISSN：0921-8777, 1992年, 273卷, 2期, 231-236页

摘要

Photolyase genes of Saccharomyces cerevisiae and Escherichia coli were expressed in S. cerevisiae and photoreactivation in nuclei and mitochondria of the host cells was analyzed by determination of survival and petit rates. Yeast photolyase was able to repair mitochondrial DNA effectively, whereas E. coli photolyase could reduce only a small fraction of the petit rate produced by UV irradiation. Analysis using fusion between yeast photolyase and E. coli lacZ genes as well as a chimeric gene between yeast and E. coli photolyase genes suggests the importance of the protruding amino terminal region of the yeast photolyase for its transport into mitochondria. A significant similarity between the protruding amino termini of yeast photolyase and yeast uracil-DNA-glycosylase suggests a common functional importance of the terminal sequences for both DNA repair enzymes.

摘要译文

酿酒酵母和大肠杆菌的光解酶基因在酿酒酵母中表达，并且通过测定存活率和小体率来分析宿主细胞的细胞核和线粒体中的光复活。酵母光解酶能够有效地修复线粒体DNA，而大肠杆菌光解酶只能减少紫外线照射产生的小部分。使用酵母光解酶和大肠杆菌lacZ基因之间的融合以及酵母和大肠杆菌光解酶基因之间的嵌合基因的分析表明酵母光解酶的突出氨基末端区域向其转运到线粒体中的重要性。酵母光裂合酶的突出氨基末端与酵母尿嘧啶-DNA-糖基化酶之间的显着相似性表明末端序列对于两种DNA修复酶的共同功能重要性。

AkiraYasui[1];HirohikoYajima[1];TakayasuKobayashi[1];Andre P.M.Eker[2];AtsushiOikawa[1];. Mitochondrial DNA repair by photolyase[J]. Mutation Research/DNA Repair, 1992,273(2): 231-236