摘要
Recently, we reported that the addition of 10% (v/v) seminal plasma to thawing solution suppressed sperm activation (cryo-capacitation) and increased implantation rates for embryos following artificial insemination (AI). In order to clarify reproductive performance such as conception rate and litter size, in this study we performed a large-scale AI trial using frozen-spermatozoa thawed by seminal plasma-containing thawing solution at the Oita Prefectural Agriculture, Forestry and Fisheries Research Center. Sows of natural estrus were cervical artificially inseminated three times per estrus (5×109/50mL per injection). When frozen-thawed spermatozoa treated with seminal plasma were injected into the uterus, high reproductive performance was observed. These results were comparable to that of liquid semen (conception rate ; 80.2 vs. 73.9% (liquid semen), litter size ; 10.1 vs. 10.3, number of live born ; 9.2 vs. 9.5). From the basic AI study, examining whether AI by seminal plasma-containing thawing solution is efficient at a commercial level, we carried out AI using frozen-thawed spermatozoa at a pig production farm. The conception rate, litter size and the number of live born after AI with seminal plasma were found to be at a standard level similar to that of liquid semen (conception rate ; 82.4 vs. 84.2% (liquid semen), litter size ; 11.8 vs. 11.0, number of live born ; 11.1 vs. 10.4). From these results, we concluded that the 10% (v/v) seminal plasma-containing thawing solution is a beneficial tool for AI using frozen-thawed boar spermatozoa.
摘要译文
近日，编了加入10％（体积/体积）精浆解冻溶液抑制精子激活（低温获能），并以下人工授精（AI）的增加的植入率的胚胎。为了澄清繁殖性能，如受胎率和产仔数，在这项研究中，我们执行使用冷冻的精子通过精浆含血浆解冻溶液解冻大规模的AI试验在大分县农业，林业和渔业研究中心。自然发情母猪宫颈人工授精发情每三次（5×10 SUP 9 / SUP /每次注射50毫升）。当冷冻解冻精子精浆处理，分别注入子宫，高繁殖性能进行了观察。这些结果相媲美的液体精液（受胎率; 80.2与73.9％（液体精液），产仔数; 10.1与10.3，活产仔数; 9.2与9.5）。从基本的AI研究，检查AI由精液含血浆解冻的解决方案是否有效率在商业层面上，我们进行了AI用冻融精子在生猪生产的农场。受胎率，产仔数和活人工智能与精浆后出生的数量被认为是在一个标准的水平类似于液体精液（受胎率; 82.4与84.2％（液体精液），产仔数; 11.8与11.0，活产仔数; 11.1与10.4）。从这些结果，我们的结论是，10％（V / V）精含血浆解冻溶液是用于人工智能使用冻融公猪精子的有益的工具。
Tetsuji OKAZAKI[1]; Teiichi AKIYOSHI[1]; Masakazu KAN[1]; Hisanori TESHIMA[1];Masayuki SHIMADA[2]. Artificial Insemination Trial of Frozen-Thawed Boar Spermatozoa with Thawing Solution Containing Seminal Plasma[J]. Nihon Yoton Gakkaishi, 2011,48(4): 164-168