摘要
The influence of progesterone on myelin of the brain in adult male Wistar rats was investigated by labelling the myelin of neurons in 5 mm thick brain sections with Nile blue stain. The following nuclei were analysed hypothalamic nucleus arcuatus (ARC) and nucleus paraventricularis (NPV) claustrum (CL), nuclei of the corticomedial part of amygdala: nucleus medialis (NM), nucleus corticalis (NCO) and nucleus centralis (NCE) and in the basolateral part of amygdala, nucleus basolateralis (NBL), nucleus basomedialis (NBM) and nucleus lateralis posterior (NLP). In control male rats sacrificed at 62 days of age a great number of neurons labelled with Nile blue for myelin were detected by stereological analysis.They were observed in; ARC and NPV, in the corticomedial amygdaloid nuclei (NM, NCE NCO) as well as in the basolateral nuclei (NBL, NBM and NLP). In CL there was a smaller number of neurons with labelled myelin than in the other investigated regions. In comparison to the controls, the number of neurons labelled with Nile blue for myelin in progesterone treated male rats was significantly reduced in ARC of hypothalamus and in NCO of amygdala. A significant increase was observed in NPV of hypothalamus, and in NM, NCE NBL and NBM of amygdala. On the other hand, in CL the number of neurons labelled with Nile blue for myelin was not changed.
摘要译文
孕酮对大脑中的成年雄性Wistar大鼠髓鞘的影响通过用尼罗蓝染色标记的神经元的髓鞘5毫米厚的脑切片的影响。下面细胞核进行分析下丘脑弓状核（ARC）和室旁核（NPV）屏状核（CL），杏仁核的corticomedial部分的核：核内侧（NM），核corticalis（NCO）和细胞核centralis（NCE）和杏仁核的基底外侧部分，核basolateralis（NBL），核basomedialis（NBM）和细胞核外侧后（NLP）。在控制雄性大鼠牺牲在62天的年龄在观察神经元标记与尼罗河蓝色的髓鞘通过体视analysis.They检测大量; ARC和NPV，在corticomedial杏仁核（NM，NCE NCO），以及在基底外侧核（NBL，NBM和NLP）。在CL有神经元比其它调查区域标记髓磷脂的数量较少。相比于对照，神经元孕酮治疗雄性大鼠标记尼罗蓝对髓鞘的数量在丘脑ARC和杏仁核中的NCO被显著降低。一个显著的增加，观察下丘脑的NPV，并在新墨西哥州，NCE NBL和杏仁核的NBM。另一方面，在CL神经元标记的尼罗蓝对髓鞘的数目没有变化。
Đelić Dijana J., Lozanče Olivera, Nikolić Zora Č., Blagojević Zdenka M., Mrvić-Jovičić Verica M., Zorić Zoran D., Drekić Dmitar M.. Changes in myelinisation of neurons in different brain regions in progesterone-treated rats[J]. Acta Veterinaria, 2003,53(5-6): 367-