摘要
Testis nuclei of hypophysectomized rats selectively accumulate labeled testosterone and 5α-dihydrotestosterone following the injection of tritiated testosterone in vivo. Testosterone and 5α-dihydrotestoste rone are bound to macromolecules in nuclei and can be extracted with 0.5 M KCl. Accumulation of protein bound radioactive androgens in nuclei of isolated seminiferous tubules is similar to that of whole testis. The relative amounts of testosterone and dihydrotestosterone in purified nuclei were similar to the relative amounts bound to cytoplasmic receptors, suggesting that cytoplasmic androgen-receptor complexes may be transported into the nuclei. Binding of labeled androgen is saturable and inhibited by prior injection of unlabeled testosterone or cyproterone acetate. Nuclear binding sites are destroyed by the proteolytic enzyme pronase, but not by DNase. Like the cytoplasmic androgen-receptor complexes in rat testis, nuclear androgen-protein complexes are heat labile and dissociate slowly at 0° C. Androgens fail to accumulate in testis nuclei of the Stanley-Gumbreck androgen insensitive rat, a species lacking cytoplasmic androgen receptors in testis and other androgen target tissues.
摘要译文
垂体切除大鼠的睾丸核在体内注射氚标记的睾酮后选择性地累积标记的睾酮和5α-二氢睾酮。睾酮和5α-二氢睾酮可与核中的大分子结合，并可用0.5M KCl萃取。分离的曲细精管细胞核中蛋白质结合放射性雄激素的积累与整个睾丸相似。纯化细胞核中睾酮和二氢睾酮的相对量与细胞质受体结合的相对量相似，表明细胞质雄激素受体复合物可能被转运到细胞核中。标记的雄激素的结合是可饱和的并且通过预先注射未标记的睾酮或醋酸环丙孕酮来抑制。核结合位点被蛋白水解酶链霉蛋白酶破坏，但不是DNase。与大鼠睾丸中的细胞质雄激素 - 受体复合物一样，核雄激素 - 蛋白质复合物是热不稳定的并且在0℃下缓慢解离。雄激素不能在Stanley-Gumbreck雄激素不敏感大鼠的睾丸核中积累，该大鼠是睾丸和其他雄激素靶组织中缺乏细胞质雄激素受体的物种。
Albert A.Smith[a][b][c];William S.McLean[a][b][c];VidarHansson[a][b][c][∗];Shihadeh N.Nayfeh;Frank S.French;. Androgen receptor in nuclei of rat testis[J]. Steroids, 1975,25(5): 569-586