摘要
Abstract
Purpose: This study aimed to determine microRNA (miRNA) expression profile of human retinal pigment epithelium cell (ARPE-19) against the oxidative stress induced by hydrogen peroxide (H2O2).
Methods: ARPE-19 cells were incubated with different concentrations of H2O2 (200, 600 and 800 μM) for 18 h, and then cell viability, vascular endothelial growth factor levels and total oxidant status were evaluated. Expressions of 1152 miRNA were determined by quantitative real-time PCR in each group.
Results: Expressions of 90 miRNA were significantly changed in the ARPE-19 cells incubated with H2O2 compared to control group. However, miR-143-3p was only found to be expressed in groups incubated with H2O2. While 24 miRNA (hsa-miR-200c-3p, miR-192-5p, miR-194-5p, miR-141-3p, miR-658, miR-18 b-5p, miR-486-5p, miR-525-3p, miR-493-3p, miR-518d-3p, miR-29 b-1-5p, miR-675-3p, miR-1238-3p, miR-195-3p, miR-1539, miR-490-5p, miR-3200-5p, miR-1273d, miR-130a-5p, miR-30 b-5p, miR-1247-5p, miR-1910-5p, miR27a-5p and miR-200 b-3p) upregulated due to the increased dose of H2O2, nine miRNA (hsa-miR-96-5p, miR-33a-5p, miR-345-5p, miR-106 b-3p, miR-1285-3p, miR-23 b-5p, miR-27 b-5p, miR-103a-3p and miR-4289) were also found to be downregulated.
Conclusion: This study suggests that oxidative stress may be an important factor on expression of miRNAs in ARPE-19 cells. These miRNAs may have a role in the pathogenesis of age-related macular degeneration related to oxidative stress. However, this relationship needs to be examined in new studies by evaluation of pathways and target genes.
摘要译文
目的：本研究旨在确定人视网膜色素上皮细胞（ARPE-19）对过氧化氢（H 2 O 2）诱导的氧化应激的microRNA（miRNA）表达谱。方法：将ARPE-19细胞与不同浓度的H 2 O 2（200,600和800μM）一起孵育18 h，然后培养细胞活力，评估血管内皮生长因子水平和总氧化剂状态。通过每组中的定量实时PCR确定1152个miRNA的表达。结果：与对照组相比，用H 2 O 2孵育的ARPE-19细胞中90个miRNA的表达显着改变。然而，仅发现miR-143-3p在与H 2 O 2温育的组中表达。 24个miRNA（hsa-miR-200c-3p，miR-192-5p，miR-194-5p，miR-141-3p，miR-658，miR-18b-5p，miR-486-5p，miR-525） -3p，的miR-493-3p，的miR-518D-3P，的miR-29 B-1-5p，的miR-675-3p，的miR-1238-3p，的miR-195-3p，的miR-1539，的miR-490-5p，的miR MiR-130a-5p，miR-30b-5p，miR-1247-5p，miR-1910-5p，miR27a-5p和miR-200 b-3p）由于H 2 O 2，9种miRNA（hsa-miR-96-5p，miR-33a-5p，miR-345-5p，miR-106 b）的剂量增加而上调-3p，miR-1285-3p，miR-23b-5p，miR-27b-5p，还发现miR-103a-3p和miR-4289）被下调。结论：本研究表明，氧化应激可能是ARPE-19细胞中miRNA表达的重要因素。这些miRNA可能在与氧化应激相关的年龄相关性黄斑变性的发病机理中起作用。然而，需要通过评估途径和靶基因在新研究中检验这种关系。