摘要
Conservation of genetic variability is one of the main goals in animal production science and the analysis of breeds genetic asset can supply objective basis for effective conservation programs and selection strategies. Bionda Piemontese(PIB) and Bianca di Saluzzo (SAB) chicken breeds originated in Piemonte region. Breeds conservation programmes started in 1999 in Verzuolo (CN) aiming to preserve the breeds and to improve their diffusion being particularly adapted to freerange rearing systems thanks to their resistance. PIB and SAB are both suggested for traditional recipes and production (e.g. Morozzo capon) and are Slow Food presidia. A total of 76 birds were analysed: PIB (n=36), SAB (n=40). Genomic DNA was extracted from blood samples. All birds were genotyped at eight microsatellite loci. Each marker was subjected to PCRand the products were separated by electrophoresis in 4.2% denaturing polyacrylamide gels on ABI Prism 377 DNA Sequencerequipped with Genescan and Genotyper software. The results of this preliminary study highlight the genetic differences occurring between PIB and SAB populations
摘要译文
内生变异性是动物生产科学的主要目标之一，品种遗传资源的分析可以为有效的保护计划和选择策略提供客观依据。Piemontese Bionda（PIB）和Bianca di Saluzzo（SAB）鸡品种起源于皮埃蒙特地区。保育计划于1999年开始在Verzuolo（CN）开展，旨在保护这些品种并提高其扩散能力，特别适用于自由放养的饲养系统。PIB和SAB都建议用于传统配方和生产（例如Morozzo capon），并且是Slow Food presidia。共分析了76只鸡：PIB（n \x3d 36），SAB（n \x3d 40）。从血液样品中提取基因组DNA。所有的鸟类在8个微卫星位点进行基因分型。对每个标记进行PCR并且通过电泳分离产物4。ABI Prism 377 DNA Sequence上的2％变性聚丙烯酰胺凝胶，配备Genescan和Genotyper软件。这项初步研究的结果突出了PIB和SAB群体之间的遗传差异
Guidobono Cavalchini[1];S.P Marelli[2];M.G. Strillacci[1];M.C. Cozzi[1];M. Polli[1];M. Longeri[1]. Heterozygosity analysis of Bionda Piemontese and Bianca di Saluzzo chicken breeds by microsatellites markers: a preliminary study[J]. Italian Journal of Animal Science, 2007,6(Sup1): 63-65