摘要
Cryptococcus neoformans and Cryptococcus gattii are encapsulated yeast agents of cryptococcosis and facultative intracellular pathogens. The interaction of these yeasts with macrophages is essential for containing the infection. However, Cryptococcus spp. overcome this initial host defense barrier using a unique pathogenic strategy involving intracellular replication and cytoplasmic accumulation of polysaccharide-containing vesicles. Here, we employed representational difference analysis (RDA) to identify C. neoformans and C. gattii genes differentially expressed during intracellular growth in rat peritoneal macrophages. The upregulated transcripts of C. neoformans during macrophage interaction were related to ATP-binding cassette (ABC) transporters, intra-golgi transport, chaperone activity, ribosomal maintenance, NAD metabolism, histone methylation, stress response, and monosaccharide metabolism. In contrast, with C. gattii, upregulated genes were associated with cell growth, aerobic respiration, protein binding, microtubule nucleation, monosaccharides and nitrogen metabolism, inositol or phosphatidylinositol phosphatase activity, cellular signaling, and stress response. Our findings reveal new genes that may be necessary for the intracellular parasitism of C. neoformans and C. gattii.
摘要译文
新型隐球菌（Cryptococcus neoformans）和加利福尼亚隐球菌（Cryptococcus gattii）是隐球菌病和兼性细胞内病原体的包封酵母剂。这些酵母与巨噬细胞的相互作用对于包含感染是必不可少的。然而，Cryptococcus spp。利用涉及含多糖囊泡的细胞内复制和细胞质积累的独特致病策略来克服该初始宿主防御屏障。这里，我们采用代表性差异分析（RDA）来鉴定大鼠腹腔巨噬细胞在细胞内生长过程中差异表达的C.neoformans和C.gattii基因。在巨噬细胞相互作用过程中，新生隐球菌的上调转录物与ATP结合盒（ABC）转运蛋白，高尔基体内转运，伴侣蛋白活性，核糖体维持，NAD代谢，组蛋白甲基化，应激反应和单糖代谢。相反，与C. gattii，上调基因与细胞生长，有氧呼吸，蛋白质结合，微管成核，单糖和氮代谢，肌醇或磷脂酰肌醇磷酸酶活性，细胞信号传导和应激反应。我们的研究结果揭示新的基因，可能是必要的细胞内寄生C.新生隐球菌和C. gattii。
Letícia Goulart; Lívia Kmetzsch Rosa E Silva; Laura Chiapello; Carolina Silveira; Juliana Crestani; Diana Masih; Marilene Henning Vainstein. Cryptococcus neoformans and Cryptococcus gattii genes preferentially expressed during rat macrophage infection[J]. Medical Mycology, 2010,48(7): 932–941