期刊文献

Fertilization and embryo recovery rates in superovulated Chios ewes after laparoscopic intrauterine insemination 收藏

腹腔镜宫腔内人工授精后超排卵希俄斯母羊的受精率和胚胎恢复率

原文求助发布源

作者

A.G.Lymberopoulos[a];G.S.Amiridis[b];B.Kühholzer[c];U.Besenfelder[d];V.Christodoulou[e];E.Vainas[a];G.Brem[d];

作者单位

[a]NAGREF-Veterinary Research Institute, GR-570 08 Ionia, Thessaloniki, Macedonia, Greece[b]Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, GR-43100 Karditsa, Greece[c]LBI fuer zyto-, immuno- und Molekulargenetische Forschung, Vienna, Austria[d]Institute of Animal Breeding and Genetics, University of Animal Science, Vienna, Austria[e]NAGREF-Institute of Animal Livestock, Paralimni, GR-581 00 Giannitsa, Pella, Greece[a]Correspondence and reprint requests:

关键词

sheep; laparoscopy; superovulation; intrauterine insemination; embryo recovery;

关键词译文

羊;腹腔镜检查;超数排卵;子宫内受精;胚胎恢复;

发布日期

1 June 2001

页码

1855-1862

DOI

10.1016/S0093-691X(01)00527-1

来源信息

Theriogenology ISSN：0093-691X, 2001年, 55卷, 9期, 1855-1862页

摘要

Forty superovulated dairy ewes of the Greek Chios breed were used in an experiment to evaluate the efficiency of laparoscopic intrauterine insemination on fertilization and embryo recovery rates as well as embryo quality. Estrus was synchronized by intravaginal progestagen impregnated sponges and superovulation was induced by administration of 8.8 mg o-FSH im following a standard 8 dose protocol. A small volume (0.3mL) of diluted fresh ram semen was deposited in each uterine horn 24 to 28 h after onset of the estrus by a laparoscopic technique. The animals were allocated randomly into two groups (Group A and B) of 20 animals each. In Group A, embryos were recovered 18 to 24 h after the intrauterine insemination and in Group B on Day 6. The average number of corpora lutea was 12.8 ± 1.2 and 11.5 ± 1.1 (± SEM); the overall embryo recovery was 66.4% and 57% and the percentage of recovered fertilized ova was 81% and 82.8% in Groups A and B, respectively. More fertilized ova were collected per ewe from Group A (P ≤ 0.1). Results indicated that in Chios breed, superovulation using homologous FSH combined with laparoscopic AI leads to good ovarian response with satisfactory results in fertilization, embryo recovery and quality of embryos. This could lead to improved and more efficient methods for obtaining large numbers of high quality oocytes and embryos for embryo transfer programs which could contribute to genetic improvement and increase of the population size.

摘要译文

希腊Chios品种的奶牛母羊被用于实验来评估腹腔镜子宫内授精对受精和胚胎恢复率以及胚胎质量的效率。通过阴道内孕激素浸渍的海绵同步发情，通过按照标准8剂量方案施用8.8mg o-FSH im诱导超排卵。小容量（0。3mL）稀释后的鲜羊精液通过腹腔镜技术在发情后24〜28 h分别沉积于子宫角。将动物随机分配到两组（A组和B组），每组20只动物。 A组胚胎在宫腔内人工授精18〜24 h后收集胚胎，B组于第6天收集胚胎。黄体平均数为12.8±1.2和11.5±1.1（±SEM）;总体胚胎恢复率为66.4％和57％，回收的受精卵比例分别为81％和82％。A组和B组分别占8％。每只母羊从A组采集更多的受精卵（P≤0.1）。结果表明，在希俄斯品种，使用同源FSH联合腹腔镜AI的超排卵导致良好的卵巢反应，在受精，胚胎恢复和胚胎质量方面具有令人满意的结果。更有效的方法获得大量的高质量卵母细胞和胚胎的胚胎移植计划，这可能有助于遗传改良和人口规模的增加。

A.G.Lymberopoulos[a];G.S.Amiridis[b];B.Kühholzer[c];U.Besenfelder[d];V.Christodoulou[e];E.Vainas[a];G.Brem[d];. Fertilization and embryo recovery rates in superovulated Chios ewes after laparoscopic intrauterine insemination[J]. Theriogenology, 2001,55(9): 1855-1862