摘要
The baculovirus expression vector system has emerged as the system of choice for the expression of a number of heterologous genes of both prokaryotic and eukaryotic origin. This system utilizes the baculovirus very late, hyperactive polyhedrin and p10 promoters to drive the transcription of foreign genes. Regulation of transcription from these promoters is presently not well understood even though a number of viral gene products that may be important for transcription have been identified. Fresh insight into host-virus interactions during baculovirus pathogenesis is now offered by the identification of insect host factors that interact with transcriptionally essential motifs of these promoters as well as cis-acting enhancer-like elements upstream from the promoter.
摘要译文
杆状病毒表达载体系统已经成为表达多种原核和真核起源的异源基因的选择系统。该系统利用杆状病毒非常晚期的高度活跃的多角体蛋白和p10启动子来驱动外源基因的转录。目前对这些启动子的转录调控尚不清楚，即使已经鉴定了许多对转录而言可能重要的病毒基因产物。通过鉴定与这些启动子的转录必需基序相互作用的昆虫宿主因子以及启动子上游的顺式作用增强子样元件，提供了对杆状病毒发病过程中宿主 - 病毒相互作用的新洞察。
Seyed E. Hasnain; Anjali Jain; Saman Habib; Sudip Ghosh; Udayan Chatterji; Aruna Ramachandran; Parimal Das; Betapudi Venkaiah; Seema Pandey; Bufeng Liang[2]; Akash Ranjan[3]; K. Natarajan[4]; C.Anser Azim[5]. Involvement of host factors in transcription from baculovirus very late promoters - a review ☆[J]. Gene, 1997,190(1): 113–118