摘要
The present study aimed to compare the fertility of ewes intrauterinally inseminated with frozen-thawed semen using a soybean-based semen extender (AndroMed) with those of ewes intrauterinally inseminated with frozen-thawed semen using a Tris-based extender containing either egg yolk or BSA. Suffolk ewes (n=104) were treated with an intravaginal sponge containing 40 mg fluoroprogesterone acetate (FGA) for 12 days and an intramuscular injection of 500 IU equine chorionic gonadotropin to induce estrus and ovulation during the non-breeding season (July, 2007). Intrauterine insemination was carried out 40-46 h after removal of the FGA sponge (n=90), regardless of the incidence of estrus. The pregnancy rates were not significantly different among the semen extenders containing egg yolk (64.5%) or BSA (58.6%) and AndroMed extender (56.7%). The lambing rates (64.5, 55.2 and 56.7% for the semen extenders containing egg yolk, BSA and AndroMed, respectively) and prolificacy (1.59 to 1.75) were also not significantly different. The present results indicate that an egg yolk-containing semen extender can be replaced with the non-animal derived extender AndroMed, which could be used for intrauterine insemination using frozen-thawed ram semen without reducing fertility.
摘要译文
使用大豆为基础的精液扩展（AndroMed）与母羊人工授精intrauterinally使用任何含有蛋黄或BSA的TRIS基于扩展冷冻精液解冻蔷薇解冻的精液。含有40mg fluoroprogesterone乙酸乙酯（FGA）为12天，在注射500 IU马绒毛膜促性腺激素肌内在非繁殖季节诱导发情和排卵（七月，2007年）。宫腔内人工授精进行，无论发情的发病率从40-46ħ去除FGA海绵（N \x3d 90）之后。怀孕率不含有蛋黄（64.5％）或BSA（58.6％）和AndroMed扩展（56.7％），精液中扩展不同显著。产羔率（64.5，55.2和56。7％的含蛋黄，BSA和AndroMed，分别）和繁殖力（1.59〜1.75）精液扩展也没有显著不同。本结果表明，精液增量鸡蛋含有蛋黄可以换成非动物来源的扩展AndroMed，这可以用冻融公羊精液不降低生育用于宫腔内人工授精。
Yutaka FUKUI[1]; Hirohide KOHNO[2]; Tetsuro TOGARI[3]; Mami HIWASA[1];Kentaro OKABE[2]. Fertility after Artificial Insemination Using a Soybean-Based Semen Extender in Sheep[J]. Journal of Reproduction and Development, 2008,54(4): 286-289