摘要
The effects of activin A and/or follistatin on the development of bovine embryos were investigated. Presumptive zygotes matured and fertilized in vitro were cultured in a chemically defined medium (modified synthetic oviduct fluid medium; mSOF). Addition of 1–100 ng/ml of activin A to mSOF significantly increased the percentage of zygotes that developed to morulae and blastocysts (48–54% and 31–41%, respectively) compared with no addition (41% and 25%, respectively). In contrast, addition of 1–100 ng/ml follistatin significantly reduced the percentage of zygotes developing to morulae and blastocysts (29–31% and 17–20%, respectively) compared with no addition (41% and 28%, respectively). In a culture with 10 ng/ml of activin A, supplementation with the same concentration of follistatin neutralized the positive effect of activin A, while supplementation with 100 ng/ml of follistatin reduced the percentage of zygotes that developed. The total cell numbers in morulae and blastocysts were not affected by the addition of activin A and/or follistatin. The development-enhancing effects of activin A and the development-impeding effects of follistatin were observed when embryos were exposed to activin A or follistatin at a concentration of 10 ng/ml prior to the 9- to 16-cell stage. These results suggest that activin A and follistatin may affect bovine embryos until the third cell cycle and may play important roles in regulation of the developmental competence of bovine embryos.
摘要译文
研究了激活素A和/或卵泡抑素对牛胚胎发育的影响。在化学成分确定的培养基（改进的合成输卵管液培养基; mSOF）中培养假定的受精卵受精并在体外受精。向mSOF中添加1-100ng / ml的激活素A显着增加发育为桑e胚和囊胚的受精卵的百分比（48-54％和31-41％分别）和不添加（分别为41％和25％）。相反，与未添加相比，添加1-100ng / ml卵泡抑素显着降低合子发育成桑e胚和囊胚的百分比（分别为29-31％和17-20％）（分别为41％和28％分别）。在含有10ng / ml活化素A的培养物中，补充相同浓度的促滤泡素抑制剂中和活化素A的积极作用，而补充100ng / ml卵泡抑素则降低了发育中受精卵的百分比。添加活化素A和/或卵泡抑素不会影响桑e胚和囊胚中的细胞总数。在9至16细胞期之前，当胚胎暴露于浓度为10ng / ml的激活素A或卵泡抑素时，观察到激活素A的cts和卵泡抑素的发育阻碍作用。这些结果表明，激活素A和卵泡抑素可能影响牛胚胎直到第三个细胞周期，并可能在牛胚胎发育能力的调节中发挥重要作用。
Koji Yoshioka;Chie Suzuki;Shokichi Iwamura. Activin A and Follistatin Regulate Developmental Competence of In Vitro-Produced Bovine Embryos[J]. Biology of Reproduction, 1998,59(5): 1017–1022