摘要
A semen extender made with low density lipoproteins (LDL) has been used instead of a standard extender that is already available on the market for the cryopreservation of bovine semen. However, in order to extend its use to artificial insemination centres, in vivo fertility studies were required. Semen was taken from three bulls and frozen-thawed in two extenders: the LDL extender and a standard Tris-egg-yolk (20%) extender used by AI centres. The quality of the semen was assessed prior to artificial insemination: motility was assessed using an image analyser (Computer Assisted Semen Analysis (Hamilton Thorne)), and the integrity of the plasma membrane was assessed using the hypo-osmotic test (HOS test). For the first time, gestations were obtained following the artificial insemination of cows in the field (n = 193) with semen that had been frozen-thawed in the LDL extender. No significant difference (p 0.05) was detected between the success rates of AI between the semen that had been frozen-thawed in the LDL extender (59.2%) and the control extender, Tris-20% egg yolk (65.3%). In conclusion, the in vivo fertility of semen that has been frozen-thawed in the LDL extender is maintained since gestations are obtained following AI.
摘要译文
用低密度脂蛋白（LDL）制成的精液填充剂已经被用来代替市场上已有的用于牛精液冷冻保存的标准补充剂。然而，为了将其用于人工授精中心，需要进行体内生育研究。精液从三头公牛中取出，并用两种稀释剂冷冻解冻：LDL填充剂和AI中心使用的标准Tris-蛋黄（20％）填充剂。在人工授精之前评估精液的质量：使用图像分析仪（计算机辅助精液分析（Hamilton Thorne））评估运动性，并使用低渗试验（HOS试验）评估质膜的完整性。首次，孕妇在田间（n \x3d 193）用精液在LDL延长剂中冷冻融化后人工授精获得了妊娠。没有显着差异（P 0。05）在低密度脂蛋白延长剂（59.2％）冷冻 - 解冻精液与对照稀释剂，Tris-20％蛋黄（65.3％）之间的AI成功率之间检测到。结论是，因为在AI后获得了妊娠，所以已经在LDL延长剂中冷冻 - 融化的精液的体内生育力得以维持。
L.Amirat-Briand[a];D.Bencharif[a];O.Vera-Munoz[a];S.Pineau[a];C.Thorin[a];S.Destrumelle[a];S.Desherces[b];M.Anton[c];M.Jouan[d];E.Shmitt[b];D.Tainturier[a];. In vivo fertility of bull semen following cryopreservation with an LDL (low density lipoprotein) extender: Preliminary results of artificial inseminations[J]. Animal Reproduction Science, 2010,122(3-4): 282-287