摘要
The present experiment was conducted to clarify the effect of bovine oviductal epithelial cells (BOEC) collected from oviducts ipsilateral to cystic follicles (CFs) using an in vitro coculture system on the development of in vitro matured/fertilized (IVM/IVF) bovine embryos. In the first comparison, the effect of the presence of CF on the development of the embryos cocultured with BOEC derived from the cows with CF (n=18) and corpus hemorrhagicum (CH, n=10) was examined. In the second comparison, the effect of the type of cyst [progesterone (P4)-dominant; n=9, estradiol-17β (E2)-dominant; n=5] on the development of the embryos cocultured with BOEC derived from the cystic cows was examined. No difference was observed between CF and CH (control) groups in the mean developmental rates of embryos developed to ≥2-cell (86.3% vs. 86.4%), 8–16 cells (53.0% vs. 56.2%), blastocyst (24.2% vs. 24.8%) and hatched blastocyst (12.0% vs. 14.6%). However, the blastocyst production rate was significantly different (P
摘要译文
使用体外成熟/受精（IVM / IVF）牛胚胎发育的体外共培养体系，从输卵管同侧收集囊状上皮细胞（BOEC）至囊性卵泡（CF）。在第一个比较中，研究了存在CF对来自具有CF（n \x3d 18）和出血性尿液（CH，n \x3d 10）的奶牛来源的BOEC共培养的胚胎发育的影响。在第二个比较中，囊肿类型[孕酮（P 4） - 优势;雌二醇-17β（E 2） - 主语;n \x3d 5]对与来自囊性牛的BOEC共培养的胚胎的发育进行了检查。在发育至≥2细胞的胚胎的平均发育率（86.3％对86.4％），8-16个细胞（53.0％对56.2％），囊胚（24.2％），CF和CH（对照）组之间没有观察到差异％与24。8％）和孵化囊胚（12.0％比14.6％）。然而，囊胚产生率有显着差异（P
HKamishita;MTakagi;Y.HChoi[1];M.P.BWijayagunawardane;KMiyazawa;KSato;. Development of in vitro matured and fertilized bovine embryos cocultured with bovine oviductal epithelial cells obtained from oviducts ipsilateral to cystic follicles[J]. Animal Reproduction Science, 1999,56(3-4): 201-209