摘要
Intrauterine insemination by laparoscopy is required to achieve acceptable lambing rates in ewes when using frozen semen but the procedure has evoked welfare concerns. Oxytocin has been used to dilate the cervix as a means of accessing the uterus during conventional cervical insemination, but its effect on fertility is not well documented. Three hundred crossbred ewes were synchronised in estrus and randomly allocated to one of three insemination procedures using frozen/thawed semen containing 400×106/ml progressively motile sperm: single cervical (0.2 ml), multiple cervical (4×0.05 ml) or laparoscopic (0.05 ml per uterine horn). The effects of each insemination procedure on lambing rate (percentage of treated ewes lambing) and litter size (lambs per ewe lambing) were tested with and without oxytocin (10 IU given i.m.) prior to fixed-time insemination. Oxytocin did not permit complete cervical penetration in any ewes and neither lambing rate nor litter size was influenced by the number of inseminations. Lambing percentages were 69 and 42 (P<0.01) for the laparoscopic and cervical insemination methods, respectively, and oxytocin reduced these to 58 (NS) and 10 (P<0.001) percent, respectively. Corresponding litter sizes for ewes not receiving oxytocin were 1.91 and 1.51 and for those receiving oxytocin, 1.83 and 1.41 (laparoscopic versus cervical, P<0.02). Thus, in the absence of complete cervical penetration at insemination, 10 IU oxytocin decreased the number of ewes lambing but had no effect on their litter size.
摘要译文
当使用冷冻精液时，需要通过腹腔镜进行子宫内授精以达到母羊可接受的产羔率，但是该程序引起了福利问题。在传统的子宫颈授精过程中，催产素已被用来扩张子宫颈作为进入子宫的一种手段，但是其对生育力的影响还没有很好的记录。红色母羊在发情期间同步并随机分配到使用含有400×106 / ml渐进活动精子的冷冻/解冻精液的三个授精程序之一：单个子宫颈（0。2毫升），多次宫颈（4×0.05毫升）或腹腔镜（每子宫角0.05毫升）。用催产素和不用催产素（每天给予10IU）测试每个授精程序对产羔率（处理的母羊产羔百分率）和产仔量（每母羊产羔数）的影响。）在定期授精之前。催产素不允许任何母羊完全的宫颈渗透，并且产羔率和产仔数都不受供精数量的影响。Lambing分别为69和42（P 0.01）分别为腹腔镜和宫颈授精方法，催产素分别减少到58（NS）和10（P 0.001）％。未接受催产素的母羊的窝产仔数分别为1.91和1.51，接受催产素的分别为1.83和1.41（腹腔镜和子宫颈，P 0.02）。从而，在人工授精时没有完全宫颈渗透的情况下，10IU催产素减少母羊产羔数量，但对其产仔量没有影响。
M.E.King[a];W.A.C.McKelvey[b];W.S.Dingwall[b];K.P.Matthews[c];F.E.Gebbie[a];M.J.A.Mylne[c];E.Stewart[b];J.J.Robinson[a];. Lambing rates and litter sizes following intrauterine or cervical insemination of frozen/thawed semen with or without oxytocin administration[J]. Theriogenology, 2004,62(7): 1236-1244