摘要
The genomic RNA (gRNA) of Pea enation mosaic virus 2 (PEMV2) is the template for p33 and −1 frameshift product p94. The PEMV2 subgenomic RNA (sgRNA) encodes two overlapping ORFs, p26 and p27, which are required for movement and stability of the gRNA. Efficient translation of p33 requires two of three 3’ proximal cap-independent translation enhancers (3’CITEs): the kl-TSS, which binds ribosomes and engages in a long-distance interaction with the 5’end; and the adjacent eIF4E-binding PTE. Unlike the gRNA, all three 3’CITEs were required for efficient translation of the sgRNA, which included the ribosome-binding 3’TSS. A hairpin in the 5’ proximal coding region of p26/p27 supported translation by the 3’CITEs by engaging in a long-distance RNA:RNA interaction with the kl-TSS. These results strongly suggest that the 5’ ends of PEMV2 gRNA and sgRNA connect with the 3’UTR through similar long-distance interactions while having different requirements for 3’CITEs.
摘要译文
豌豆入侵花叶病毒2（PEMV2）的基因组RNA（gRNA）是p33和-1移码产物p94的模板。 PEMV2亚基因组RNA（sgRNA）编码两个重叠的ORF，p26和p27，这是gRNA的运动和稳定性所需的。 p33的高效翻译需要三个3''''近端帽独立的翻译增强子（3''''CITE）中的两个：k1-TSS，其结合核糖体并参与与5''''端的长距离相互作用;和相邻的eIF4E绑定PTE。与gRNA不同，所有三个3''''CITE都需要有效翻译sgRNA，其中包括核糖体结合3''''TSS。在p26 / p27的5''''近端编码区中的发夹通过参与与kl-TSS的长距离RNA：RNA相互作用来支持3''''CITE的翻译。这些结果强烈表明，PEMV2 gRNA和sgRNA的5''''末端通过类似的长距离相互作用与3''''UTR连接，同时对3''''CITE具有不同的要求。
FengGao;Anne E.Simon;. Differential use of 3’CITEs by the subgenomic RNA of Pea enation mosaic virus 2[J]. Virology, 2017,510: 194-204