期刊文献

Comparison of plant- and egg yolk-based semen diluents on in vitro sperm kinematics and in vivo fertility of frozen-thawed bull semen 收藏

基于植物和蛋黄的精液稀释液在冷冻 - 解冻公牛精液体外精子运动和体内生育力中的比较

原文求助发布源

作者

E.M.Murphy[a][b];C.O’Meara[b];B.Eivers[b];P.Lonergan[c];S.Fair[a];

作者单位

[a]Laboratory of Animal Reproduction, Department of Biological Sciences, School of Natural Sciences, Faculty of Science and Engineering, University of Limerick, Limerick, V94 T9PX, Ireland[b]National Cattle Breeding Centre, Naas, Co Kildare, W91 WF59, Ireland[c]School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, D04 N2E5, Ireland

关键词

Sperm; Frozen-thawed semen; Diluents; Artificial insemination; Non-return rates;

关键词译文

精子;冷冻解冻精液;稀释剂;人工授精;不退货率;

发布日期

1 April 2018

页码

70-75

DOI

10.1016/j.anireprosci.2018.02.010

来源信息

Animal Reproduction Science ISSN：0378-4320, 2018年, 191卷, 70-75页

摘要

Diluents using components of plant origin have been developed as an alternative to animal based extenders for the dilution of bull semen, however, it is unclear if use of these diluents results in in vivo fertility rates similar to those that occur with use of traditional egg yolk-based diluents. The aim of this study was to assess the effect of semen diluent on 60-day non-return rate (NRR) following artificial insemination (AI) with frozen-thawed bull semen. The effect of semen dilution in one of three different commercial diluents (BullXcell – egg yolk-based, OptiXcell – plant-based or AndroMed – plant-based) on post-thaw total and progressive motility as well as kinematic parameters (Experiment 1) and field fertility (Experiment 2, n = 1,480 inseminations) was assessed. Semen stored in OptiXcell had greater post-thaw total and progressive motility than AndroMed (P P P > 0.05). There was no difference in any other sperm kinematic parameters (P > 0.05). There was no effect of diluent on 60-day NRR (71.5%, 67.8% and 70.6% for BullXcell, OptiXcell and AndroMed, respectively). In conclusion, while diluent significantly affected post-thaw sperm motility and kinematics, no effect on 60-day NRR was observed. Given that OptiXcell and AndroMed are animal protein-free media these diluents may be a suitable alternative to BullXcell for the storage of frozen-thawed bull semen.

摘要译文

使用植物来源的组分的稀释剂已经被开发作为公牛精液稀释的基于动物的稀释剂的替代物，然而，目前还不清楚这些稀释剂的使用是否导致与使用传统蛋黄基稀释剂时相似的体内生育率。本研究的目的是评估人工授精（AI）后冷冻解冻公牛精液对精液稀释液对60天不归还率（NRR）的影响。在三种不同的商业稀释剂（BullXcell-基于蛋黄的，（实验1）和田间肥力（实验2，n \x3d 1，实验1），对于解冻后总的和渐进的运动性，OptiXcell-基于植物或基于AndroMed-480次授精）进行了评估。存储在OptiXcell中的精液比AndroMed具有更大的解冻后总和进行性运动（PPP 0.05）。其他精子运动参数无差异（P 0.05）。稀释剂对60天NRR无效（BullXcell，OptiXcell和AndroMed分别为71.5％，67.8％和70.6％）。总之，虽然稀释剂显着影响解冻后的精子活力和运动学，但未观察到对60天NRR的影响。鉴于OptiXcell和AndroMed是无动物蛋白的培养基，这些稀释剂可能是BullXcell适用于冷冻 - 解冻公牛精液储存的替代品。

E.M.Murphy[a][b];C.O’Meara[b];B.Eivers[b];P.Lonergan[c];S.Fair[a];. Comparison of plant- and egg yolk-based semen diluents on in vitro sperm kinematics and in vivo fertility of frozen-thawed bull semen[J]. Animal Reproduction Science, 2018,191: 70-75