摘要
This study investigated the effects of N, N-Dimethylglycine (DMG) on the development of in vitro produced (IVP) bovine embryos. IVP embryos were obtained by in vitro fertilization of in vitro matured oocytes for 6 h. In Experiment 1, IVP embryos were cultured in mSOFaa supplemented with bovine serum albumin but without glucose (SOF1) for 4 days, transferred to mSOFaa (with 5% fetal bovine serum and 1.5 mM glucose; SOF2) supplemented with 0 (control), 0.1,1 or 10 μM DMG and cultured for an additional 7 days (11 days in total) to assess their development in vitro. When cultured in the medium with 0.1 μM DMG, a significantly higher number of IVP embryos developed to the blastocyst and hatched blastocyst stages (40.3 and 40.8%, respectively) compared with the other groups (18.7-31.0% and 15.0-28.7%, respectively; P<0.05, analysis of variance). In Experiment 2, IVP embryos were cultured in SOF1 with or without 0.1 μM DMG for 4 days, transferred to SOF2 with or without 0.1 μM DMG and further cultured as in Experiment 1; DMG was added to either SOF1 or SOF2 and to both of them to assess its exposure effects on embryo development. When cultured continuously with DMG for 11 days, significantly higher rates of IVP embryos developed into blastocyst and hatched blastocyst stages (39.0 and 47.7%, respectively) compared with the other groups (31.0-32.2% and 29.5-31.0%, respectively; P<0.05). In Experiment 3, we examined developmental speed of IVP embryos cultured with or without addition of 0.1 μM DMG to IVC medium after 7 days of IVC. When DMG was added to IVC medium, the ratio of embryos developed to advanced developmental stages (No. of embryos developed to the blastocyst and expanded blastocyst stages/No. of embryos developed to the morula stage) was 28.7% (86/3) and 7 times higher than that of those cultured without DMG, 4.0% (52/13). These results suggest that addition of 0.1 μM DMG to mSOFaa during IVC of IVP bovine embryos has a promoting effect on their development.
摘要译文
这项研究调查了在体外产生（IVP）牛胚胎的发育N，N-二甲基甘氨酸（DMG）的效应。IVP胚胎通过体外受精体外成熟的卵母细胞得到的6小时。在实验1中，IVP胚胎中mSOFaa培养补充有牛血清白蛋白的但没有葡萄糖（SOF1）4天，转移到mSOFaa（用5％胎牛血清和1.5毫米的葡萄糖;SOF2）补充有0（对照），0.1,1或10μM的DMG和培养另外7天（共11天），以评估其在体外发育。当与0.1μMDMG的培养基中培养，一些显著高于发展到囊胚IVP胚胎和孵化囊胚阶段（分别为40.3和40.8％），与其他组相比，（18.7-31.0％和15.0-28.7％，分别; P 0.05，方差分析）。在实验2中，IVP胚胎培养中SOF1有或没有0.1微米的DMG 4天，转移到SOF2有或没有0。1μM的DMG和进一步培养如试验1; DMG加入要么SOF1或SOF2和他们两个，以评估其对胚胎发育的影响曝光。当与DMG 11天连续培养，IVP胚胎显著更高的速率发展成囊胚和孵化囊胚阶段（39.0和47.7％，分别）相比，与其他组（分别为31.0-32.2％和29.5-31.0％; P 0.05）。在实验3中，我们研究了有或没有加入0培养IVP胚胎发育速度。1μMDMG至IVC中后7天IVC的。当DMG加入IVC中，胚胎的比例发展到晚期的发育阶段（第发展到囊胚的胚胎和扩展囊胚阶段/否。发展到桑椹期）的胚胎为28.7％（三分之八十六），比那些没有培养DMG，4高出7倍。0％（52/13）。这些结果表明，加入0.1μMDMG到mSOFaa期间IVC IVP的牛胚胎对他们的发展产生促进作用。
Toshikiyo TAKAHASHI[1]; Ryu ITOH[1];Takashi NAGAI[2]. Effects of N, N-Dimethylglycine on the Development of In Vitro Produced Bovine Embryos[J]. Journal of Reproduction and Development, 2009,55(3): 339-342