摘要
A technique for laparoscopic intrauterine insemination in bitches is described. During natural estrus, 5 beagle bitches were inseminated and S others were naturally mated (control group) twice at a 48-h interval on Days 3 and S (n = 4) or Days 4 and 6 (n = 6) after the increase in plasma progesterone considered to be indicative of the day of the preovulatory LH peak. All the inseminations were with fresh semen and under general anesthesia. The technique involved the introductions of 1) a Verres needle to insufflate the abdominal cavity by direct punction on the middle line 1 cm over the umbilicus, 2) a laparoscope to visualize the abdominal cavity by a 1 cm puncture on the middle line 1 cm under the umbilicus, 3) a forceps used to manipulate the uterus by a 0.5 cm puncture at 2 to 3 cm lateral to the mammary glands, and 4) an 18-g catheter used to puncture the uterus on the middle line between the 3rd and 5th mammary gland. The uterine body was grasped by the forceps and elevated against the ventral abdominal wall. The 18-g catheter was then inserted through the abdominal wall directly into the uterine lumen, and 1.0 ml of fresh semen containing 250 to 480 × 106 spermatozoa/ml was injected. The inseminations resulted in pregnancies in all animals. Litter size was similar in the artificially inseminated and naturally mated bitches (5 ± 1.8 and 4.8 ± 1.6 pups per litter, respectively). Bitches in the artificially inseminated group delivered at 65.2 ± 0.8 d and in the natural mated group at 65.4 ± 0.5 d after the LH peak. In conclusion, this paper gives the first results of intrauterine laparoscopic insemination in bitches, indicating interesting perspectives for this technique in dog's reproduction.
摘要译文
描述了一种腹腔镜宫内授精技术。在自然发情期间，在第3天和第S天（n \x3d 4）或第4天和第6天（n \x3d 6）以48小时间隔两次后，血浆黄体酮浓度的增加被认为是排卵前LH峰值的一天。所有的授精均采用新鲜精液和全身麻醉。该技术涉及引入1）Verres针，通过在肚脐上方1cm处的中线上直接结扎来给腹腔注气，2）腹腔镜，通过在肚脐下1cm的中线上1厘米的穿刺来观察腹腔，3）用于操纵子宫的镊子，在乳腺外侧2〜3cm进行5cm穿刺，4）在第3乳腺和第5乳腺之间的中线穿刺18g导管。子宫体被镊子抓住并抬高腹腹壁。然后将18g导管通过腹壁直接插入子宫腔内，注射0ml含250-480×10 6精子/ ml的新鲜精液。授精导致所有动物怀孕。在人工授精和自然交配母犬的窝窝大小相似（分别为5±1.8和4.8±1.6个窝仔）。人工授精组为65.2±0.8 d，自然配对组为LH峰后65.4±0.5 d。结论是，本文给出了宫腔内腹腔镜授精的第一个结果，表明这种技术在狗的繁殖方面有趣的观点。
L.D.M.Silva[a];K.Onclin[a];F.Snaps[b];J.Verstegen[a];. Laparoscopic intrauterine insemination in the bitch[J]. Theriogenology, 1995,43(3): 615-623